Anti-p19INK4d Antibody (monoclonal) | Boster Immunoleader MA1075 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-p19INK4d Antibody (monoclonal)

catalog :

MA1075

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

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product information

Product Name :

Anti-p19INK4d Antibody (monoclonal)

Catalog Number :

MA1075

Clonality :

Monoclonal (Clone: IMD-19)

Description short :

Mouse IgG monoclonal antibody for p19INK4d, cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) (CDKN2D) detection. Tested with WB, IHC-P, ICC in Human. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human

Applications :

WB,IHC-P,ICC

Western blot :

At 0.5-1μg/ml with the appropriate system to detect p19INK4d in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 1-2μg/ml to detect p19INK4d in formalin fixed and paraffin embedded tissues.

Immunocytochemistry :

Suitable

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Recombinant human p19INK4d.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and ICC.

Background :

Cyclins are important in regulating the cell cycle through their formation of enzymatic complexes with various cyclin-dependent kinases. P19(INK4d) also kowns as cyclin-dependent kinase inhibitor 2D, is one of the novel members of the mouse INK4 gene family. Okuda et al.(1995) described the cloning of the human INK4d gene(CDKN2D). The predicted 166-amino acid protein is 86% identical to the mouse protein and about 45% identical to other human INK4 family members.

Reference :

1. Hirai, H.; Roussel, M. F.; Kato, J.-Y.; Ashmun, R. A.; Sherr, C. J. : Novel INK4 proteins, p19 and p18, are specific inhibitors of cyclin D-dependent kinases CDK4 and CDK6. Molec. Cell. Biol. 15: 2672-2681, 1995. 2. tsuzaki, Y.; Miyazawa, K.; Yokota, T.; Hitomi, T.; Yamagishi, H.; Sakai, T. : Molecular cloning and characterization of the human p19(INK4d) gene promoter. FEBS Lett. 517: 272-276, 2002.

Gene Name :

CDKN2D

Protein Name :

Cyclin-dependent kinase 4 inhibitor D

Gene Full Name :

cyclin-dependent kinase inhibitor 2D(p19, inhibits CDK4)

Synonyms :

INK4D p19 p19-INK4d

Uniprot ID :

Human(P55273), Mouse(Q60773)

more info or order :

Boster Immunoleader product webpage