Anti-p16INK4a/CDKN2 Antibody (monoclonal) | Boster Immunoleader MA1074 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-p16INK4a/CDKN2 Antibody (monoclonal)

catalog :

MA1074

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - frozen section

publications citing this reagent :

Wu C, Huang T, Xie Q, Wu D, Xu X. Construction of pETNF-P16 plasmid and its expression properties in EC9706 cell line induced by X-ray irradiation. World J Gastroenterol. 2004;10:2927-30 pubmed

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-p16INK4a/CDKN2 Antibody (monoclonal)

Catalog Number :

MA1074

Clonality :

Monoclonal (Clone: IMD-16)

Description short :

Mouse IgG monoclonal antibody for p16INK4a/CDKN2, cyclin-dependent kinase inhibitor 2A (CDKN2A) detection. Tested with WB, IHC-F, ICC in Human. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human

Applications :

WB,IHC-F,ICC

Western blot :

At 0.5-1μg/ml with the appropriate system to detect P16 in cells and tissues.

Immunohistochemistry(Frozen Section) :

At 1-2μg/ml to detect P16 in formalin or acetone fixed tissues.

Immunocytochemistry :

Suitable

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Recombinant human p16 protein.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(F) and ICC.

Background :

p16(INK4A), also known as cyclin-dependent kinase inhibitor 2A(CDKN2A), or multiple tumor suppressor 1(MTS1). The p16 gene(CDKN2A) was mapped to 9p21. The p16 gene encodes a negative regulator of the cell cycle. CDKN2 plays an important role during tumorigenesis or tumor progression in a significant proportion of pancreatic adenocarcinomas. Germ-line mutations in the CDKN2A tumor-suppressor gene have been linked to the development of melanoma in some families with inherited melanoma.

Reference :

1. Stone, S.; Jiang, P.; Dayananth, P.; Tavtigian, S. W.; Katcher, H.; Parry, D.; Peters, G.; Kamb, A. : Complex structure and regulation of the p16(MTS1) locus. Cancer Res. 55: 2988-2994, 1995. 2. Monzon, J.; Liu, L.; Brill, H.; Goldstein, A. M.; Tucker, M. A.; From, L.; McLaughlin, J.; Hogg, D.; Lassam, N. J. : CDKN2A mutations in multiple primary melanomas. New Eng. J. Med. 338: 879-887, 1998. 3. Bartsch, D.; Shevlin, D. W.; Tung, W. S.; Kisker, O.; Wells, S. A., Jr.; Goodfellow, P. J. : Frequent mutations of CDKN2 in primary pancreatic adenocarcinomas. Genes Chromosomes Cancer 14: 189-195, 1995

Gene Name :

CDKN2A

Protein Name :

Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3

Gene Full Name :

cyclin-dependent kinase inhibitor 2A

Synonyms :

ARF MLM P14 P16 P19 CMM2 INK4 MTS1 TP16 CDK4I CDKN2 INK4A MTS-1 P14ARF P19ARF P16INK4 P16INK4A P16-INK4A CDK4 INHIBITOR cyclin-dependent kinase inhibitor 2A(melanoma, p16, inhibits CDK4) Cyclin-dependent kinase 4 inhibitor A Multiple tumor suppressor 1 Collaborator of ARF

Uniprot ID :

Human(P42771), Mouse(P51480), Rat(Q9R0Z3)

more info or order :

Boster Immunoleader product webpage