Boster Immunoleader

antibody

Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (monoclonal)

MA1072

100 ug/vial

200 USD

monoclonal

mouse

human, mouse, rat

Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (monoclonal)

MA1072

Monoclonal (Clone: N1)

Mouse IgG monoclonal antibody for Nitric Oxide Synthase, Brain (1-181) NOS1, nitric oxide synthase 1 (neuronal) (NOS1) detection. Tested with WB in Human;rat;goat;pig. No cross reactivity with other proteins.

200 USD

Mouse IgG1

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Human, goat, pig, rat

WB

At 0.5μg/ml with the appropriate system to detect Nitric Oxide Synthase, Brain(1-181) in cells and tissues.

Other applications have not been tested. Optimal dilutions should be determined by end users.

Recombinant neuronal NOS fragment(amino acids 1-181) from rat brain.

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Ascites

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB.

Nitric Oxide Synthase 1(NOS1,neuronal NOS,nNOS1) is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. It may be involved in neuronal cell death and damage in neurological illness. nNOS1 localized to the 12q24.2 region of human chromosome 12. It splice variant, expressed in testis, that encodes an NH2-terminal truncated protein of 1098 amino acids. nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2. Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300 bp of each other but separated from exon 2 by an intron that is at least 20 kb in length. The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle. The novel gaseous neuromediator nitric oxide is though to play an important role in development and plasticity. Despite this, gene-knockout mice lacking neuronal(Type I) nitric oxide synthase exhibit relatively normal brain development and behavior.

1. Brenman, J. E.; Chao, D. S.; Xia, H.; Aldape, K.; Bredt, D. S. : Nitric oxide synthase complexed with dystrophin and absent from skeletal muscle sarcolemma in Duchenne muscular dystrophy. Cell 82: 743-752, 1995. 2. Wang, Y.; Goligorsky, M. S.; Lin, M.; Wilcox, J. N.; Marsden, P. A. : A novel, testis-specific mRNA transcript encoding an NH(2)-terminal truncated nitric-oxide synthase. J. Biol. Chem. 272: 11392-11401, 1997. 3. Xie, J.; Roddy, P.; Rife, T. K.; Murad, F.; Young, A. P. : Two closely linked but separable promoters for human neuronal nitric oxide synthase gene transcription. Proc. Nat. Acad. Sci. 92: 1242-1246, 1995. 4. Kharazia, V. N.; Schmidt, H. H. H. W.; Weinberg, R. J. : Type I nitric oxide synthase fully accounts for NADPH-diaphorase in rat striatum, but not cortex. Neuroscience 62: 983-987, 1994.

NOS1

Nitric oxide synthase, brain

nitric oxide synthase 1(neuronal)

NOS bNOS nNOS IHPS1 N-NOS NC-NOS Neuronal NOS Peptidyl-cysteine S-nitrosylase NOS1

Human(P29475), Mouse(Q9Z0J4), Rat(P29476)