Anti-NF200 Antibody (monoclonal) | Boster Immunoleader MA1071 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-NF200 Antibody (monoclonal)

catalog :

MA1071

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

publications citing this reagent :

Wang J, Zeng Y, Wu J, Li Y, Teng Y. Cograft of neural stem cells and schwann cells overexpressing TrkC and neurotrophin-3 respectively after rat spinal cord transection. Biomaterials. 2011;32:7454-68 pubmed publisher
Zhang W, Yan Q, Zeng Y, Zhang X, Xiong Y, Wang J, et al. Implantation of adult bone marrow-derived mesenchymal stem cells transfected with the neurotrophin-3 gene and pretreated with retinoic acid in completely transected spinal cord. Brain Res. 2010;1359:256-71 pubmed publisher
Xue Q, Luan X, Gu Y, Wu H, Zhang G, Yu G, et al. The negative co-signaling molecule b7-h4 is expressed by human bone marrow-derived mesenchymal stem cells and mediates its T-cell modulatory activity. Stem Cells Dev. 2010;19:27-38 pubmed publisher
Zeng Y, Zhang X, Wang J, Zhang W, Chen S. Combination of adenoviral vector-mediated neurotrophin-3 gene transfer and retinoic acid promotes adult bone marrow cells to differentiate into neuronal phenotypes. Neurosci Lett. 2006;408:98-103 pubmed

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-NF200 Antibody (monoclonal)

Catalog Number :

MA1071

Clonality :

Monoclonal (Clone: NF-200)

Description short :

Mouse IgG monoclonal antibody for NF200, neurofilament, heavy polypeptide (NEFH) detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, mouse, rat

Applications :

WB,IHC-P,IHC-F

Western blot :

At 0.5ml with the appropriate system to detect NF200 in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 1-2μg/ml to detect NF200 in formalin fixed and paraffin embedded tissues.

Immunohistochemistry(Frozen Section) :

At 1-2μg/ml to detect NF200 in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and IHC(F).

Background :

Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD(NFL), 125 kD(NFM), and 200 kD(NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein(NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions(exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease(CMT).

Reference :

1. Lees, J. F.; Shneidman, P. S.; Skuntz, S. F.; Carden, M. J.; Lazzarini, R. A. : The structure and organization of the human heavy neurofilament subunit (NF-H) and the gene encoding it. EMBO J. 7: 1947-1955, 1988. 2. Brownlees, J.; Ackerley, S.; Grierson, A. J.; Jacobsen, N. J. O.; Shea, K.; Anderton, B. H.; Leigh, P. N.; Shaw, C. E.; Miller, C. C. J. : Charcot-Marie-Tooth disease neurofilament mutations disrupt neurofilament assembly and axonal transport. Hum. Molec. Genet. 11: 2837-2844, 2002.

Gene Name :

NEFH

Protein Name :

Neurofilament heavy polypeptide

Gene Full Name :

neurofilament, heavy polypeptide

Synonyms :

NFH KIAA0845 200 kDa neurofilament protein Neurofilament triplet H protein

Uniprot ID :

Human(P12036), Mouse(P19246), Rat(P16884)

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