Anti-NF68 Antibody (monoclonal) | Boster Immunoleader MA1070 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-NF68 Antibody (monoclonal)

catalog :

MA1070

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-NF68 Antibody (monoclonal)

Catalog Number :

MA1070

Clonality :

Monoclonal (Clone: NF-68)

Description short :

Mouse IgG monoclonal antibody for NF68, neurofilament, light polypeptide (NEFL ) detection. Tested with WB, IHC-P, IHC-F in Human;pig;rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, pig, rat

Applications :

WB,IHC-P,IHC-F

Western blot :

At 1-2μg/ml with the appropriate system to detect NF68 in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 2-4μg/ml to detect NF68 in formalin fixed and paraffin embedded tissues.

Immunohistochemistry(Frozen Section) :

At 2-4μg/ml to detect NF68 in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Pig spinal cord.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and IHC(F).

Background :

Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD(NFL), 125 kD(NFM) and 200 kD(NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. Neurofilament 68(NF68), also called Neurofilament Protein, Light Chain(NFL). It is one of the most abundant cytoskeletal components of the neuron. Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E(CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons.

Reference :

1. Zhu, Q.; Couillard-Despres, S.; Julien, J.-P. : Delayed maturation of regenerating myelinated axons in mice lacking neurofilaments. Exp. Neurol. 148: 299-316, 1997. 2. Jordanova, A.; De Jonghe, P.; Boerkoel, C. F.; Takashima, H.; De Vriendt, E.; Ceuterick, C.; Martin, J.-J.; Butler, I. J.; Mancias, P.; Papasozomenos, S. C.; Terespolsky, D.; Potocki, L.; Brown, C. W.; Shy, M.; Rita, D. A.; Tournev, I.; Kremensky, I.; Lupski, J. R.; Timmerman, V. : Mutations in the neurofilament light chain gene (NEFL) cause early onset severe Charcot-Marie-Tooth disease. Brain 126: 590-597, 2003. 3. Previtali, S. C.; Zerega, B.; Sherman, D. L.; Brophy, P. J.; Dina, G.; King, R. H. M.; Salih, M. M.; Feltri, L.; Quattrini, A.; Ravazzolo, R.; Wrabetz, L.; Monaco, A. P.; Bolino, A. : Myotubularin-related 2 protein phosphatase and neurofilament light chain protein, both mutated in CMT neuropathies, interact in peripheral nerve. Hum. Molec. Genet. 12: 1713-1723, 2003.

Gene Name :

NEFL

Protein Name :

Neurofilament light polypeptide

Gene Full Name :

neurofilament, light polypeptide

Synonyms :

NFL NF-L NF68 CMT1F CMT2E 68 kDa neurofilament protein Neurofilament triplet L protein

Uniprot ID :

Human(P07196), Mouse(P08551), Rat(P19527)

more info or order :

Boster Immunoleader product webpage