Anti-N-Cadherin Antibody (monoclonal) | Boster Immunoleader MA1067 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-N-Cadherin Antibody (monoclonal)

catalog :

MA1067

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-N-Cadherin Antibody (monoclonal)

Catalog Number :

MA1067

Clonality :

Monoclonal (Clone: NC-17)

Description short :

Mouse IgG monoclonal antibody for N-Cadherin, cadherin 2, type 1, N-cadherin (neuronal) (CDH2 ) detection. Tested with WB, IHC-F in Human;mouse;rat;rabbit;chicken. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, mouse, rat, rabbit, chicken

Applications :

WB,IHC-F

Western blot :

At 2μg/ml with the appropriate system to detect N-cadherin in cells and tissues.

Immunohistochemistry(Frozen Section) :

At 4μg/ml to detect N-cadherin in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Affinity purified chicken heart A-CAM.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(F).

Background :

N-cadherin(NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule(L-CAM). N-Cadherin,, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96% identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene(including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.

Reference :

1. Miyatani, S.; Copeland, N. G.; Gilbert, D. J.; Jenkins, N. A.; Takeichi, M. : Genomic structure and chromosomal mapping of the mouse N-cadherin gene. Proc. Nat. Acad. Sci. 89: 8443-8447, 1992. 2. Walsh, F. S.; Barton, C. H.; Putt, W.; Moore, S. E.; Kelsell, D.; Spurr, N.; Goodfellow, P. N. : N-cadherin gene maps to human chromosome 18 and is not linked to the E-cadherin gene. J. Neurochem. 55: 805-812, 1990. 3. Togashi, H.; Abe, K.; Mizoguchi, A.; Takaoka, K.; Chisaka, O.; Takeichi, M. : Cadherin regulates dendritic spine morphogenesis. Neuron 35: 77-89, 2002.

Gene Name :

CDH2

Protein Name :

Cadherin-2

Gene Full Name :

cadherin 2, type 1, N-cadherin(neuronal)

Synonyms :

CDHN NCAD CD325 CDw325 Neural cadherin

Uniprot ID :

Human(P19022), Mouse(P15116), Rat(Q9Z1Y3)

more info or order :

Boster Immunoleader product webpage