product summary
request information :
company name :
Boster Immunoleader
product type :
antibody
product name :
Anti-Myosin(Smooth) Antibody (monoclonal)
catalog :
MA1065
quantity :
100 ug/vial
price :
200 USD
clonality :
monoclonal
host :
mouse
reactivity :
human, mouse, rat, chicken
application :
western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
Product Name :
Anti-Myosin(Smooth) Antibody (monoclonal)
Catalog Number :
MA1065
Clonality :
Monoclonal (Clone: MS-13)
Description short :
Mouse IgG monoclonal antibody for Myosin(Smooth), myosin, heavy chain 11, smooth muscle (MYH11 ) detection. Tested with WB, IHC-P, IHC-F in Human;rabbit;chicken. No cross reactivity with other proteins.
Price :
200 USD
Ig type :
Mouse IgG1
Size :
100 ug/vial
Form :
Lyophilized
Specificity :
No cross reactivity with other proteins.
Reactivity :
Human, chicken, rabbit
Applications :
WB,IHC-P,IHC-F
Western blot :
At 0.25-0.5μg/ml with the appropriate system to detect myosin(smooth) in cells and tissues.
Immunohistochemistry(Paraffin-embedded S ... :
At 0.5-1μg/ml to detect myosin(smooth) in formalin fixed and paraffin embedded tissues.
Immunohistochemistry(Frozen Section) :
At 0.5-1μg/ml to detect myosin(smooth) in formalin or acetone fixed tissues.
Notes :
Other applications have not been tested. Optimal dilutions should be determined by end users.
Immunogen :
Human uterus smooth muscle extract.
Contents :
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Purification :
Ascites
Reconstitution :
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
Storage :
At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.
Relevant detection systems :
Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and IHC(F).
Background :
Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. Southern blots of a panel of hybrids containing different portions of human chromosome 16 localized the gene to 16p13.13-p13.12.
Reference :
1. Deng, Z.; Liu, P.; Marlton, P.; Claxton, D. F.; Lane, S.; Callen, D. F.; Collins, F. S.; Siciliano, M. J. : Smooth muscle myosin heavy chain locus (MYH11) maps to 16p13.13-p13.12 and establishes a new region of conserved synteny between human 16p and mouse 16. Genomics 18: 156-159, 1993. 2. Khau Van Kien, P.; Mathieu, F.; Zhu, L.; Lalande, A.; Betard, C.; Lathrop, M.; Brunotte, F.; Wolf, J.-E.; Jeunemaitre, X. : Mapping of familial thoracic aortic aneurysm/dissection with patent ductus arteriosus to 16p12.2-p13.13 Circulation 112: 200-206, 2005.
Gene Name :
MYH11
Protein Name :
Myosin-11
Gene Full Name :
myosin, heavy chain 11, smooth muscle
Synonyms :
AAT4 FAA4 SMHC SMMHC KIAA0866 Myosin heavy chain 11 Myosin heavy chain, smooth muscle isoform
Uniprot ID :
Human(P35749), Mouse(O08638), Rat(Q63862)
company information
Boster Immunoleader
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
www.bosterbio.com
925.485.4527
headquarters: USA
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