Anti-P-Glycoprotein(MDR) Antibody (monoclonal) | Boster Immunoleader MA1060 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-P-Glycoprotein(MDR) Antibody (monoclonal)

catalog :

MA1060

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-P-Glycoprotein(MDR) Antibody (monoclonal)

Catalog Number :

MA1060

Clonality :

Monoclonal (Clone: PG-13)

Description short :

Mouse IgG monoclonal antibody for P-Glycoprotein (MDR), ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1 ) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human

Applications :

WB,IHC-P,IHC-F,ICC

Western blot :

At 0.5-1μg/ml with the appropriate system to detect MDR in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 1-2μg/ml to detect MDR in formalin fixed and paraffin embedded tissues. Boiling the sections is required.

Immunohistochemistry(Frozen Section) :

At 1-2μg/ml to detect MDR in formalin or acetone fixed tissues.

Immunocytochemistry :

Suitable

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Mixture of human and hamster drug-resistant whole cells and crude plasma membranes.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P), IHC(F) and ICC.

Background :

P-Glycoprotein,, also known as Multidrug Resistance 1(MDR1), is one of the ATP-binding cassette transporters family. P-glycoprotein-1 is involved in the transport of 3 of these protease inhibitors in vitro. MDR1 gene is mapped to the 7q21.1 by in situ hybridization. The MDR1 gene product, P-glycoprotein, mediates the transport of the cardiac glycoside, digoxin.

Reference :

1. Callen, D. F.; Baker, E.; Simmers, R. N.; Seshadri, R.; Roninson, I. B. : Localization of the human multiple drug resistance gene, MDR1, to 7q21.1. Hum. Genet. 77: 142-144, 1987. 2. de Lannoy, I. A. M.; Silverman, M. : The MDR1 gene product, P-glycoprotein, mediates the transport of the cardiac glycoside, digoxin. Biochem. Biophys. Res. Commun. 189: 551-557, 1992.

Gene Name :

ABCB1

Protein Name :

Multidrug resistance protein 1

Gene Full Name :

ATP-binding cassette, sub-family B(MDR/TAP), member 1

Synonyms :

CLCS MDR1 P-GP PGY1 ABC20 CD243 GP170 ATP-binding cassette sub-family B member 1;P-glycoprotein 1

Uniprot ID :

Human(P08183), Mouse(P21447), Rat(P43245)

more info or order :

Boster Immunoleader product webpage