Boster Immunoleader

antibody

Anti-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Antibody (monoclonal)

MA1055

100 ug/vial

200 USD

monoclonal

mouse

human, mouse, rat

western blot, immunocytochemistry, immunohistochemistry - paraffin section

Anti-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Antibody (monoclonal)

MA1055

Monoclonal (Clone: IL-13)

Mouse IgG monoclonal antibody for MAP Kinase, Activated (Diphosphorylated ERK-1&2), mitogen-activated protein kinase 1; mitogen-activated protein kinase 3 (MAPK1; MAPK3 ) detection. Tested with WB, IHC-P, ICC in Human;mouse;rat;yeast. No cross reactivity with other proteins.

200 USD

Mouse IgG1

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Human, mouse, rat, yeast

WB,IHC-P,ICC

At 0.25-0.5μg/ml with the appropriate system to detect MAP kinase, activated (Diphosphorylated ERK-1&2) in cells and tissues.

At 0.4-1μg/ml to detect MAP kinase, activated (Diphosphorylated ERK-1&2) in formalin fixed and paraffin embedded tissues.

Suitable

Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide containing 11 amino acids, HTGFLTpEYpVAT, corresponding to the phosphorylated form of ERK-activation loop conjugated to KLH.

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Ascites

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and ICC.

In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein(MAP) kinase pathways. The central core of each MAP kinase(MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase(MAPKKK) phosphorylates and activates a specific MAPK kinase(MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome.

1. Rodriguez-Viciana, P.; Tetsu, O.; Tidyman, W. E.; Estep, A. L.; Conger, B. A.; Santa Cruz, M.; McCormick, F.; Rauen, K. A. : Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome. Science 311: 1287-1290, 2006. 2. Scholl, F. A.; Dumesic, P. A.; Barragan, D. I.; Harada, K.; Bissonauth, V.; Charron, J.; Khavari, P. A. : Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Dev. Cell 12: 615-629, 2007.

MAPK1; MAPK3

Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3

mitogen-activated protein kinase 1; mitogen-activated protein kinase 3

ERK p38 p40 p41 ERK2 ERT1 MAPK2 PRKM1 PRKM2 P42MAPK p41mapk ERK1 ERT2 ERK-1 PRKM3 P44ERK1 P44MAPK HS44KDAP HUMKER1A p44-ERK1 p44-MAPK Extracellular signal-regulated kinase 2 MAP kinase isoform p42 Mitogen-activated protein kinase 2

Human(P28482), Mouse(P63085), Rat(P63086), Human(P27361), Mouse(Q63844), Rat(P21708)