Anti-Laminin Antibody (monoclonal) | Boster Immunoleader MA1054 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Laminin Antibody (monoclonal)

catalog :

MA1054

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Laminin Antibody (monoclonal)

Catalog Number :

MA1054

Clonality :

Monoclonal (Clone: LAM-26)

Description short :

Mouse IgG monoclonal antibody for Laminin, laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, beta 2 (laminin S) (LAMA1; LAMA2; LAMB1; LAMB2) detection. Tested with WB, IHC-P in Human;feline;pig. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, feline, pig

Applications :

WB,IHC-P

Western blot :

At 0.25-0.5μg/ml with the appropriate system to detect laminin in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 0.5-1μg/ml to detect laminin formalin fixed and paraffin embedded tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Human laminin.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P).

Background :

Laminin is a heterotrimeric extracellular matrix protein consisting of 3 chains: alpha-1,beta-1 and gamma-1, formerly called beta-2(LAMA2). This gene is over 260,000 base pairs and contains 64 exons. Laminin is similar with merosin, a basement membrane-associated protein found in placenta, striated muscle, and peripheral nerve, and both of them are members of the same family of basement membrane proteins. And merosin is the same as laminin M, a striated muscle-specific, basal-lamina-associated protein, it may play a primary role in the pathogenesis of that disorder.

Reference :

1. Zhang, X.; Vuolteenaho, R.; Tryggvason, K. : Structure of the human laminin alpha-2-chain gene (LAMA2), which is affected in congenital muscular dystrophy. J. Biol. Chem. 271: 27664-27669, 1996. 2. Ehrig, K.; Leivo, I.; Argraves, W. S.; Ruoslahti, E.; Engvall, E. : Merosin, a tissue-specific basement membrane protein, is a laminin-like protein. Proc. Nat. Acad. Sci. 87: 3264-3268, 1990. 3. Arahata, K.; Hayashi, Y. K.; Mizuno, Y.; Yoshida, M.; Ozawa, E. : Dystrophin-associated glycoprotein and dystrophin co-localisation at sarcolemma in Fukuyama congenital muscular dystrophy. (Letter) Lancet 342: 623-624, 1993.

Gene Name :

LAMA1; LAMA2; LAMB1; LAMB2

Protein Name :

Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2

Gene Full Name :

laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, beta 2(laminin S)

Synonyms :

LAMA Laminin a LAMM CLM LIS5 LAMB1 LAMS NPHS5 Laminin M chain Laminin-12 subunit alpha Laminin-2 subunit alpha Laminin-4 subunit alpha Merosin heavy chain S-LAM beta

Uniprot ID :

Human(P25391), Mouse(P19137), Rat(D4A409), Human(P24043), Mouse(Q60675), Rat(F1M614), Human(P07942), Mouse(P02469), Rat(D3ZQN7), Human(P55268), Mouse(Q61292), Rat(P15800)

more info or order :

Boster Immunoleader product webpage