Anti-Involucrin Antibody (monoclonal) | Boster Immunoleader MA1053 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Involucrin Antibody (monoclonal)

catalog :

MA1053

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Involucrin Antibody (monoclonal)

Catalog Number :

MA1053

Clonality :

Monoclonal (Clone: IL-9)

Description short :

Mouse IgG monoclonal antibody for Involucrin, involucrin (IVL) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;dog;pig. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, pig

Applications :

WB,IHC-P,IHC-F,ICC

Western blot :

At 2μg/ml with the appropriate system to detect involucrin in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 0.4-1μg/ml to detect involucrin in formalin fixed and paraffin embedded tissues.

Immunohistochemistry(Frozen Section) :

At 0.4-1μg/ml to detect involucrin in formalin or acetone fixed tissues.

Immunocytochemistry :

Suitable

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Human involucrin.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P), IHC(F) and ICC.

Background :

Involucrin is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. The gene consists of 585 amino acids, 390 of which form a central decapeptide repeat, rich in glutamine and glutamic acid. The involucrin gene, encoding a protein of the terminally differentiated keratinocyte, is polymorphic in the human. Involucrin has previously been mapped to chromosome 1q21. The epidermal protein involucrin has been remodeled in the higher primates. And the existence of two populations of East Asian involucrin alleles gives support for the existence of a Eurasian stem lineage from which Caucasians and a part of the Asian population originated.

Reference :

1. Eckert, R. L.; Green, H. : Structure and evolution of the human involucrin gene. Cell 46: 583-589, 1986. 2. Djian, P.; Delhomme, B.; Green, H. : Origin of the polymorphism of the involucrin gene in Asians. Am. J. Hum. Genet. 56: 1367-1372, 1995.

Gene Name :

IVL

Protein Name :

Involucrin

Gene Full Name :

involucrin

Uniprot ID :

Human(P07476), Mouse(P48997), Rat(P48998)

more info or order :

Boster Immunoleader product webpage