Anti-Insulin Antibody (monoclonal) | Boster Immunoleader MA1052 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Insulin Antibody (monoclonal)

catalog :

MA1052

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

immunohistochemistry - paraffin section

publications citing this reagent :

Liu L, Gu H, Zhao Y, An L, Yang J. Glypican 4 may be involved in the adipose tissue redistribution in high-fat feeding C57BL/6J mice with peroxisome proliferators-activated receptor γ agonist rosiglitazone treatment. Exp Ther Med. 2014;8:1813-1818 pubmed
Zhang Y, Ye C, Wang G, Gao Y, Tan K, Zhuo Z, et al. Kidney-targeted transplantation of mesenchymal stem cells by ultrasound-targeted microbubble destruction promotes kidney repair in diabetic nephropathy rats. Biomed Res Int. 2013;2013:526367 pubmed publisher
Shen L, Liu X, Hou W, Yang G, Wu Y, Zhang R, et al. NDRG2 is highly expressed in pancreatic beta cells and involved in protection against lipotoxicity. Cell Mol Life Sci. 2010;67:1371-81 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Insulin Antibody (monoclonal)

Catalog Number :

MA1052

Clonality :

Monoclonal (Clone: ISL-8J)

Description short :

Mouse IgG monoclonal antibody for Insulin, insulin (INS) detection. Tested with IHC-P in Human;mouse;rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, rabbit, rat

Applications :

IHC-P

Immunohistochemistry(Paraffin-embedded S ... :

At 0.4-1μg/ml to detect insulin in formalin fixed and paraffin embedded tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Human insulin.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by SA1021 in IHC(P).

Background :

Insulin, synthesized by the beta cells of the islets of Langerhans, consists of 2 dissimilar polypeptide chains, A and B, which are linked by 2 disulfide bonds. The insulin gene contains 3 exons and 2 introns; exon 2 encodes the signal peptide, the B chain, and part of the C peptide, while exon 3 encodes the remainder of the C peptide and the A chain. Localization of the human insulin gene to the distal end of the short arm of chromosome 11. Harper et al.(1981) and Harper and Saunders(1981) assigned the insulin gene to 11p15.5 by in situ hybridization.

Reference :

1. Harper, M. E.; Ullrich, A.; Saunders, G. F. : Localization of the human insulin gene to the distal end of the short arm of chromosome 11. Proc. Nat. Acad. Sci. 78: 4458-4460, 1981. 2. Owerbach, D.; Bell, G. I.; Rutter, W. J.; Shows, T. B. : The insulin gene is located on chromosome 11 in human. Nature 286: 82-84, 1980. 3. Huerre, C.; Gilgenkrantz, S.; Leonard, C.; Pictet, R.; Kaplan, J. C.; Junien, C. : Regional assignment of the structural gene for insulin to 11p15.1-11p15.5 by deletion mapping. (Abstract) Cytogenet. Cell Genet. 37: 495, 1984.

Gene Name :

INS

Protein Name :

Insulin

Gene Full Name :

insulin

Synonyms :

PROINSULIN ILPR IRDN IDDM2 MODY10 Insulin2 Ins-2

Uniprot ID :

Human(P01308), Mouse(P01326), Rat(P01323)

more info or order :

Boster Immunoleader product webpage