Anti-HSP70 Antibody (monoclonal) | Boster Immunoleader MA1050 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-HSP70 Antibody (monoclonal)

catalog :

MA1050

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat, cow

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

publications citing this reagent :

Ouyang W, Gao F, Wang L, Xie X, Lei F, Zhou J, et al. Thermoseed hyperthermia treatment of mammary orthotopic transplantation tumors in rats and impact on immune function. Oncol Rep. 2010;24:973-9 pubmed
Peng M, Chen M, Ling N, Xu H, Qing Y, Ren H. Novel vaccines for the treatment of chronic HBV infection based on mycobacterial heat shock protein 70. Vaccine. 2006;24:887-96 pubmed

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-HSP70 Antibody (monoclonal)

Catalog Number :

MA1050

Clonality :

Monoclonal (Clone: SJ-70)

Description short :

Mouse IgG monoclonal antibody for HSP70, heat shock 70kDa protein 1A; heat shock 70kDa protein 1B (HSPA1A; HSPA1B) detection. Tested with WB, IHC-P, IHC-F in Human. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, bovine, rabbit, rat

Applications :

WB,IHC-P,IHC-F

Western blot :

At 0.5μg/ml with the appropriate system to detect HSP70 in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 0.5-1μg/ml to detect HSP70 in formalin fixed and paraffin embedded tissues.

Immunohistochemistry(Frozen Section) :

At 0.5-1μg/ml to detect HSP70 in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

HSP70 isolated from bovine brain.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and IHC(F).

Background :

Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex(MHC) class III region 92 kilobases(kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.

Reference :

1. Milner, C. M.; Campbell, R. D. : Structure and expression of the three MHC-linked HSP70 genes. Immunogenetics 32: 242-251, 1990. 2. Ungewickell, E. : The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles. EMBO J. 4: 3385-3391, 1985.

Gene Name :

HSPA1A; HSPA1B

Protein Name :

Heat shock 70 kDa protein 1A/1B

Gene Full Name :

heat shock 70kDa protein 1A; heat shock 70kDa protein 1B

Synonyms :

HSPA1 Hsp70-1 Hsp70-2 Heat shock 70 kDa protein 1/2

Uniprot ID :

Human(P08107), Mouse(Q61696), Rat(Q07439), Mouse(P17879)

more info or order :

Boster Immunoleader product webpage