Anti-HSP60 Antibody (monoclonal) | Boster Immunoleader MA1049 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-HSP60 Antibody (monoclonal)

catalog :

MA1049

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-HSP60 Antibody (monoclonal)

Catalog Number :

MA1049

Clonality :

Monoclonal (Clone: SJ-60)

Description short :

Mouse IgG monoclonal antibody for HSP60, heat shock 60kDa protein 1 (chaperonin) (HSPD1) detection. Tested with WB, IHC-P in Human;rat;chicken. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, chicken, rat

Applications :

WB,IHC-P

Western blot :

At 2-4μg/ml with the appropriate system to detect HSP60 in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 4-8μg/ml to detect HSP60 in formalin fixed and paraffin embedded tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Recombinant human heat shock protein 60(HSP60)

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P).

Background :

Heat shock 60KD protein(HSP60) is a member of the chaperonin class of protein factors and the nuclear encoded mitochondrial HSP60 is required for the assembly into oligomeric complexes of proteins imported into the mitochondrial matrix. HSP60 is linked head to head comprising approximately 17 kb and consist of 12 exons. HSP60 is a self-molecule, it can downregulate adaptive immune responses by upregulating Tregs innately through TLR2 signaling.

Reference :

1. Cheng, M. Y.; Hartl, F.-U.; Martin, J.; Pollock, R. A.; Kalousek, F.; Neupert, W.; Hallberg, E. M.; Hallberg, R. L.; Horwich, A. L. : Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria. Nature 337: 620-625, 1989. 2. Hansen, J. J.; Bross, P.; Westergaard, M.; Nielsen, M. N.; Eiberg, H.; Borglum, A. D.; Mogensen, J.; Kristiansen, K.; Bolund, L.; Gregersen, N. : Genomic structure of the human mitochondrial chaperonin genes: HSP60 and HSP10 are localised head to head on chromosome 2 separated by a bidirectional promoter. Hum. Genet. 112: 71-77, 2003. Note: Erratum: Hum. Genet. 112: 436 only, 2003. 3. Zanin-Zhorov, A.; Cahalon, L.; Tal, G.; Margalit, R.; Lider, O.; Cohen, I. R. : Heat shock protein 60 enhances CD4+CD25+ regulatory T cell function via innate TLR2 signaling. J. Clin. Invest. 116: 2022-2032, 2006.

Gene Name :

HSPD1

Protein Name :

60 kDa heat shock protein, mitochondrial

Gene Full Name :

heat shock 60kDa protein 1(chaperonin)

Synonyms :

SPG13 GROEL CPN60 HuCHA60 spastic paraplegia 13(autosomal dominant) CHAPERONIN, 60-KD Mitochondrial matrix protein P1 P60 lymphocyte protein Heat shock protein 60 60 kDa chaperonin Chaperonin 60

Uniprot ID :

Human(P10809), Mouse(P63038), Rat(P63039)

more info or order :

Boster Immunoleader product webpage