Anti-HSP25 Antibody (monoclonal) | Boster Immunoleader MA1048 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-HSP25 Antibody (monoclonal)

catalog :

MA1048

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-HSP25 Antibody (monoclonal)

Catalog Number :

MA1048

Clonality :

Monoclonal (Clone: SJ-25)

Description short :

Mouse IgG monoclonal antibody for HSP25, heat shock 27kDa protein 1 (HSPB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, mouse, rabbit, rat

Applications :

WB,IHC-P,IHC-F,ICC

Western blot :

At 0.5-2μg/ml with the appropriate system to detect HSP25 in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 1-2μg/ml to detect HSP25 in formalin fixed and paraffin embedded tissues. Boiling the sections may improve the staining.

Immunohistochemistry(Frozen Section) :

At 1-2μg/ml to detect HSP25 in formalin or acetone fixed tissues.

Immunocytochemistry :

Suitable

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Partially purified inhibitor of actin polymerization(IAP) protein from turkey gizzard smooth muscle.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P), IHC(F) and ICC.

Background :

The heat-shock proteins(HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Heat-shock 27-kD protein1also knows as HSPB. Synthesis of the small(27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. HSP27 gene is mapped to 7q11.23. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.

Reference :

1. Evgrafov, O. V.; Mersiyanova, I.; Irobi, J.; Van Den Bosch, L.; Dierick, I.; Leung, C. L.; Schagina, O.; Verpoorten, N.; Van Impe, K.; Fedotov, V.; Dadali, E.; Auer-Grumbach, M.; and 14 others : Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Nature Genet. 36: 602-606, 2004. 2. Wyttenbach, A.; Sauvageot, O.; Carmichael, J.; Diaz-Latoud, C.; Arrigo, A.-P.; Rubinsztein,D.C. :Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin. Hum. Molec. Genet. 11: 1137-1151, 2002.

Gene Name :

HSPB1

Protein Name :

Heat shock protein beta-1

Gene Full Name :

heat shock 27kDa protein 1

Synonyms :

HSP27 HSP28 CMT2F HMN2B 28 kDa heat shock protein Estrogen-regulated 24 kDa protein Heat shock 27 kDa protein

Uniprot ID :

Human(P04792), Mouse(P14602), Rat(P42930)

more info or order :

Boster Immunoleader product webpage