Anti-GFAP Antibody (monoclonal) | Boster Immunoleader MA1045 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-GFAP Antibody (monoclonal)

catalog :

MA1045

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

publications citing this reagent :

Zhu F, Zhang L, Ding Y, Zhao J, Zheng Y. Neonatal intrahippocampal injection of lipopolysaccharide induces deficits in social behavior and prepulse inhibition and microglial activation in rats: Implication for a new schizophrenia animal model. Brain Behav Immun. 2014;38:166-74 pubmed publisher
Ye X, Xu S, Xin Y, Yu S, Ping Y, Chen L, et al. Tumor-associated microglia/macrophages enhance the invasion of glioma stem-like cells via TGF-β1 signaling pathway. J Immunol. 2012;189:444-53 pubmed publisher
Wu L, Miao S, Zou L, Wu P, Hao H, Tang K, et al. Lipoxin A4 inhibits 5-lipoxygenase translocation and leukotrienes biosynthesis to exert a neuroprotective effect in cerebral ischemia/reperfusion injury. J Mol Neurosci. 2012;48:185-200 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-GFAP Antibody (monoclonal)

Catalog Number :

MA1045

Clonality :

Monoclonal (Clone: GA-8)

Description short :

Mouse IgG monoclonal antibody for GFAP, glial fibrillary acidic protein (GFAP) detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, pig, rat

Applications :

WB,IHC-P,IHC-F

Western blot :

At 0.5-1μg/ml with the appropriate system to detect GFAP in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 0.4-1μg/ml to detect GFAP in formalin fixed and paraffin embedded tissues.

Immunohistochemistry(Frozen Section) :

At 0.5-1μg/ml to detect GFAP in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

GFAP from pig spinal cord.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and IHC(F).

Background :

Glial fibrillary acidic protein(GFAP) is an intermediate filament protein of 52Kda. GFAP gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.

Reference :

1. Brenner, M.; Johnson, A. B.; Boespflug-Tanguy, O.; Rodriguez, D.; Goldman, J. E.; Messing, A. : Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nature Genet. 27: 117-120, 2001. 2. Rodriguez, D.; Gauthier, F.; Bertini, E.; Bugiani, M.; Brenner, M.; N'guyen, S.; Goizet, C.; Gelot, A.; Surtees, R.; Pedespan, J.-M.; Hernandorena, X.; Troncoso, M.; Uziel, G.; Messing, A.; Ponsot, G.; Pham-Dinh, D.; Dautigny, A.; Boespflug-Tanguy, O. : Infantile Alexander disease: spectrum of GFAP mutations and genotype-phenotype correlation. Am. J. Hum. Genet. 69: 1134-1140, 2001. Note: Erratum: Am. J. Hum. Genet. 69: 1413 only, 2001.

Gene Name :

GFAP

Protein Name :

Glial fibrillary acidic protein

Gene Full Name :

glial fibrillary acidic protein

Synonyms :

FLJ45472 intermediate filament protein

Uniprot ID :

Human(P14136), Mouse(P03995), Rat(P47819)

more info or order :

Boster Immunoleader product webpage