Anti-Gelsolin Antibody (monoclonal) | Boster Immunoleader MA1044 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Gelsolin Antibody (monoclonal)

catalog :

MA1044

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Gelsolin Antibody (monoclonal)

Catalog Number :

MA1044

Clonality :

Monoclonal (Clone: GEL-42)

Description short :

Mouse IgG monoclonal antibody for Gelsolin, gelsolin (GSN) detection. Tested with WB, IHC-P, IHC-F in Human; rabbit; bovine. No cross reactivity with otherproteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, rabbit

Applications :

WB,IHC-P,IHC-F

Western blot :

At 0.5-1μg/ml with the appropriate system to detect gelsolin in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 1-2μg/ml to detect gelsolin in formalin fixed and paraffin embedded tissues.

Immunohistochemistry(Frozen Section) :

At 1-2μg/ml to detect gelsolin in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Human plasma gelsolin.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and IHC(F).

Background :

Gelsolin, a protein of leukocytes, platelets, and other cells, severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A gelsolin variant with 23 more N-terminal amino acids is a plasma component probably involved in the clearance of actin, the most abundant human protein, from the circulation. Gelsolin is located in 9q34. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain.

Reference :

1. Kwiatkowski, D. J.; Ozelius, L.; Schuback, D.; Gusella, J.; Breakefield, X. O. : The gelsolin (GSN) cDNA clone, from 9q32-34, identifies BclI and StuI RFLPs. Nucleic Acids Res. 17: 4425 only, 1989. 2. Kwiatkowski, D. J.; Westbrook, C. A.; Bruns, G. A. P.; Morton, C. C. : Localization of gelsolin proximal to ABL on chromosome 9. Am. J. Hum. Genet. 42: 565-572, 1988. 3. Kwiatkowski, D. J.; Stossel, T. P.; Orkin, S. H.; Mole, J. E.; Colten, H. R.; Yin, H. L. : Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain. Nature 323: 455-458, 1986.

Gene Name :

GSN

Protein Name :

Gelsolin

Gene Full Name :

gelsolin

Synonyms :

AGEL ADF Brevin Actin-depolymerizing factor

Uniprot ID :

Human(P06396), Mouse(P13020), Rat(Q68FP1)

more info or order :

Boster Immunoleader product webpage