Anti-GAP43 Antibody (monoclonal) | Boster Immunoleader MA1042 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-GAP43 Antibody (monoclonal)

catalog :

MA1042

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-GAP43 Antibody (monoclonal)

Catalog Number :

MA1042

Clonality :

Monoclonal (Clone: GAP-8A12)

Description short :

Mouse IgG monoclonal antibody for GAP43, growth associated protein 43 (GAP43) detection. Tested with WB, IHC-P, IHC-F in Human; mouse; rat; chicken; snake. No cross reactivity with otherproteins.

Price :

200 USD

Ig type :

Mouse IgG2a

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, mouse, rat

Applications :

WB,IHC-P,IHC-F

Western blot :

At 0.5-1μg/ml with the appropriate system to detect GAP43 in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 1-2μg/ml to detect GAP43 in formalin fixed and paraffin embedded tissues.

Immunohistochemistry(Frozen Section) :

At 1-2μg/ml to detect GAP43 in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

GAP-43 from neonatal rat forebrain membranes.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and IHC(F).

Background :

GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding. GAP43 is considered to be a crucial component of an effective regenerative response in the nervous system.

Reference :

1. Kosik, K. S.; Orecchio, L. D.; Bruns, G. A. P.; Benowitz, L. I.; MacDonald, G. P.; Cox, D. R.; Neve, R. L. : Human GAP-43: its deduced amino acid sequence and chromosomal localization in mouse and human. Neuron 1: 127-132, 1988. 2. Strittmatter, S. M.; Fankhauser, C.; Huang, P. L.; Mashimo, H.; Fishman, M. C. : Neuronal pathfinding is abnormal in mice lacking the neuronal growth cone protein GAP-43. Cell 80: 445-452, 1995.

Gene Name :

GAP43

Protein Name :

Neuromodulin

Gene Full Name :

growth associated protein 43

Synonyms :

B-50 PP46 protein F1 axonal membrane protein GAP-43 calmodulin-binding protein P-57 nerve growth-related peptide GAP43 neural phosphoprotein B-50 neuron growth-associated protein 43

Uniprot ID :

Human(P17677), Mouse(P06837), Rat(P07936)

more info or order :

Boster Immunoleader product webpage