Anti-Filensin Antibody (monoclonal) | Boster Immunoleader MA1041 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Filensin Antibody (monoclonal)

catalog :

MA1041

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat, cow

application :

western blot, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Filensin Antibody (monoclonal)

Catalog Number :

MA1041

Clonality :

Monoclonal (Clone: FIL-27)

Description short :

Mouse IgG monoclonal antibody for Filensin, beaded filament structural protein 1, filensin (BFSP1) detection. Tested with WB, IHC-F in Human. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, bovine

Applications :

WB,IHC-F

Western blot :

At 1-2μg/ml with the appropriate system to detect filensin in cells and tissues.

Immunohistochemistry(Frozen Section) :

At 2-4μg/ml to detect filensin in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Human and bovine lens filament enriched fraction(plasma membrane-cytoskeleton complex).

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(F).

Background :

Filensin, also known as beaded filament structure protein 1, have two major component BFSP1 and BESP2. Filensin gene is mapped at 20p12.1-p11.23. The sequence of the predicted 665-amino acid human protein is 62% and 50% identical to those of bovine and chicken filensin, respectively. However, it has less than 26% identity to other members of the intermediate filament(IF) family.

Reference :

1. Rendtorff, N. D.; Hansen, C.; Silahtaroglu, A.; Henriksen, K. F.; Tommerup, N. : Isolation of the human beaded-filament structural protein 1 gene (BFSP1) and assignment to chromosome 20p11.23-p12.1. Genomics 53: 114-116, 1998. 2. Hess, J. F.; Casselman, J. T.; FitzGerald, P. G. : Chromosomal locations of the genes for the beaded filament proteins CP 115 and CP 47. Curr. Eye Res. 14: 11-18, 1995.

Gene Name :

BFSP1

Protein Name :

Filensin

Gene Full Name :

beaded filament structural protein 1, filensin

Synonyms :

CP115 CP94 CP115 LIFL-H LIFL-H CYTOSKELETAL PROTEIN, 115-KD Beaded filament structural protein 1 Lens fiber cell beaded-filament structural protein CP 115 Lens intermediate filament-like heavy

Uniprot ID :

Human(Q12934), Mouse(A2AMT1), Rat(Q02435)

more info or order :

Boster Immunoleader product webpage