Boster Immunoleader

antibody

Anti-Episialin, EMA Antibody (monoclonal)

MA1039

100 ug/vial

200 USD

monoclonal

mouse

human, mouse, rat

immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Anti-Episialin, EMA Antibody (monoclonal)

MA1039

Monoclonal (Clone: EMA-39)

Mouse IgG monoclonal antibody for Episialin, EMA, mucin 1, cell surface associated (MUC1) detection. Tested with IHC-P, IHC-F in Human. No cross reactivity with other proteins.

200 USD

Mouse IgG1

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Human

IHC-P,IHC-F

At 2-4μg/ml to detect EMA in formalin fixed and paraffin embedded tissues.

At 2-4μg/ml to detect EMA in formalin or acetone fixed tissues.

Other applications have not been tested. Optimal dilutions should be determined by end users.

Human milk fat globule membranes.

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Ascites

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by SA1021 in IHC(P) and IHC(F).

MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1(EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type I transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.

1. Lu, W.; Hisatsune, A.; Koga, T.; Kato, K.; Kuwahara, I.; Lillehoj, E. P.; Chen, W.; Cross, A. S.; Gendler, S. J.; Gewirtz, A. T.; Kim, K. C. : Cutting edge: enhanced pulmonary clearance of Pseudomonas aeruginosa by Muc1 knockout mice. J. Immun. 176: 3890-3894, 2006. 2. Sood, R.; Zehnder, J. L.; Druzin, M. L.; Brown, P. O. : Gene expression patterns in human placenta. Proc. Nat. Acad. Sci. 103: 5478-5483, 2006. 3. Wei, X.; Xu, H.; Kufe, D. : MUC1 oncoprotein stabilizes and activates estrogen receptor alpha. Molec. Cell 21: 295-305, 2006.

MUC1

Mucin-1

mucin 1, cell surface associated

CD227 EMA PEM PEMT KL-6 PUM MUCIN 1, URINARY Episialin H23AG PEANUT-REACTIVE URINARY MUCIN MUCIN, TUMOR-ASSOCIATED EPITHELIAL POLYMORPHIC EPITHELIAL MUCIN EPITHELIAL MEMBRANE ANTIGEN mucin 1, transmembrane Breast carcinoma-associated antigen DF3 Carcinoma-associated mucin Krebs von den Lungen-6 Peanut-reactive urinary mucin Polymorphic epithelial mucin Tumor-associated epithelial membrane antigen Tumor-associated mucin

Human(15941), Mouse(02496), Rat(B2GV31)