Anti-Dystrophin Antibody (monoclonal) | Boster Immunoleader MA1037 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Dystrophin Antibody (monoclonal)

catalog :

MA1037

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Dystrophin Antibody (monoclonal)

Catalog Number :

MA1037

Clonality :

Monoclonal (Clone: DYS-48)

Description short :

Mouse IgG monoclonal antibody for Dystrophin, dystrophin (DMD) detection. Tested with WB, IHC-P in Human;mouse;rat;chicken. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG2b

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, mouse, rabbit, rat

Applications :

WB,IHC-P

Western blot :

At 1-2μg/ml with the appropriate system to detect dystrophin in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 2-4μg/ml to detect dystrophin in formalin fixed and paraffin embedded tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Recombinant human dystrophin fragment.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P).

Background :

Dystrophin(DMD) gene has 79 exons spanning at least 2,300 kb(2.3 Mb). The C terminus of the dystrophin protein is encoded by a highly conserved, alternatively spliced region of the gene. beta-dystroglycan binding activity is expressed by the dystrophin fragment spanning amino acids 3026-3345 containing the ZZ domain. DMD transcript is formed by at least 60 exons; the first half of the transcript is formed by a minimum of 33 exons spanning nearly 1000 kb, and the remaining portion has at least 27 exons that may spread over a similar distance. Dystrophin gene is expressed at a higher level in primary cultures of neuronal cells than in astro-glial cells derived from adult mouse brain. overexpression of dystrophin prevents the development of the abnormal mechanical properties associated with dystrophic muscle without causing deleterious side effects.

Reference :

1. Chelly, J.; Hamard, G.; Koulakoff, A.; Kaplan, J.-C.; Kahn, A.; Berwald-Netter, Y. : Dystrophin gene transcribed from different promoters in neuronal and glial cells. Nature 344: 64-65, 1990. 2. Cox, G. A.; Cole, N. M.; Matsumura, K.; Phelps, S. F.; Hauschka, S. D.; Campbell, K. P.; Faulkner, J. A.; Chamberlain, J. S. : Overexpression of dystrophin in transgenic mdx mice eliminates dystrophic symptoms without toxicity. Nature 364: 725-729, 1993.

Gene Name :

DMD

Protein Name :

Dystrophin

Gene Full Name :

dystrophin

Synonyms :

BMD CMD3B DXS142 DXS164 DXS206 DXS230 DXS239 DXS268 DXS269 DXS270 DXS272

Uniprot ID :

Human(P11532), Mouse(P11531), Rat(P11530)

more info or order :

Boster Immunoleader product webpage