Anti-Desmin Antibody (monoclonal) | Boster Immunoleader MA1036 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Desmin Antibody (monoclonal)

catalog :

MA1036

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Desmin Antibody (monoclonal)

Catalog Number :

MA1036

Clonality :

Monoclonal (Clone: DES-82)

Description short :

Mouse IgG monoclonal antibody for Desmin , desmin (DES) detection. Tested with WB, IHC-P in Human;mouse;rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, mouse, rat, rabbit.

Applications :

WB,IHC-P

Western blot :

At 2μg/ml with the appropriate system to detect desmin in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 2-4μg/ml to detect desmin in formalin fixed and paraffin embedded tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Desmin from pig stomach.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P).

Background :

Desmin belongs to the type III family of intermediate filaments, a class of cytoskeletal elements. DES gene encodes desmin, a muscle-specific cytoskeletal protein found in smooth, cardiac, and heart muscles. Tidball(1992) found that desmin was codistributed with actin thin filaments within the cellular processes of myotendinous junctions in frog skeletal muscle. DES gene contains 9 exons and spans about 8.4 kb. By in situ hybridization, Viegas-Pequignot et al.(1989) localized the gene to 2q35. Desmin mutation responsible for idiopathic dilated cardiomyopathy.

Reference :

1. Tidball, J. G. : Desmin at myotendinous junctions. Exp. Cell Res. 199: 206-212, 1992. 2. Viegas-Pequignot, E.; Lin, L. Z.; Dutrillaux, B.; Apiou, F.; Paulin, D. : Assignment of human desmin gene to band 2q35 by nonradioactive in situ hybridization. Hum. Genet. 83: 33-36, 1989. 3. Li, D.; Tapscoft, T.; Gonzalez, O.; Burch, P. E.; Quinones, M. A.; Zoghbi, W. A.; Hill, R.; Bachinski, L. L.; Mann, D. L.; Roberts, R. : Desmin mutation responsible for idiopathic dilated cardiomyopathy. Circulation 100: 461-464, 1999.

Gene Name :

DES

Protein Name :

Desmin

Gene Full Name :

desmin

Synonyms :

CMD1I CSM1 CSM2 intermediate filament protein

Uniprot ID :

Human(P17661), Mouse(P31001), Rat(P48675)

more info or order :

Boster Immunoleader product webpage