Anti-Cyclin D2 Antibody (monoclonal) | Boster Immunoleader MA1034 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Cyclin D2 Antibody (monoclonal)

catalog :

MA1034

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Cyclin D2 Antibody (monoclonal)

Catalog Number :

MA1034

Clonality :

Monoclonal (Clone: CY-D2)

Description short :

Mouse IgG monoclonal antibody for Cyclin D2, cyclin D2 (CCND2) detection. Tested with WB, ICC in Human;mouse. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG2a

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, mouse

Applications :

WB,ICC

Western blot :

At 2-4μg/ml with the appropriate system to detect cyclin D2 in cells and tissues.

Immunocytochemistry :

Suitable

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Recombinant human cyclin D2 protein.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in ICC.

Background :

Inaba et al.(1992) used murine cDNA clones for 3 cyclin D genes that are normally expressed during the G1 phase of the cell cycle to clone the cognate human genes. D-type cyclins(cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. cyclin D2(CCND2) gene ia assigned to 12p13.

Reference :

1. Kozar, K.; Ciemerych, M. A.; Rebel, V. I.; Shigematsu, H.; Zagozdzon, A.; Sicinska, E.; Geng, Y.; Yu, Q.; Bhattacharya, S.; Bronson, R. T.; Akashi, K.; Sicinski, P. : Mouse development and cell proliferation in the absence of D-cyclins. Cell 118: 477-491, 2004. 2. Inaba, T.; Matsushime, H.; Valentine, M.; Roussel, M. F.; Sherr, C. J.; Look, A. T. : Genomic organization, chromosomal localization, and independent expression of human cyclin D genes. Genomics 13: 565-574, 1992. 3. Xiong, Y.; Menninger, J.; Beach, D.; Ward, D. C. : Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins. Genomics 13: 575-584, 1992.

Gene Name :

CCND2

Protein Name :

G1/S-specific cyclin-D2

Gene Full Name :

cyclin D2

Synonyms :

KIAK0002

Uniprot ID :

Human(P30279), Mouse(P30280), Rat(Q04827)

more info or order :

Boster Immunoleader product webpage