Anti-Cyclin D1 Antibody (monoclonal) | Boster Immunoleader MA1033 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Cyclin D1 Antibody (monoclonal)

catalog :

MA1033

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

publications citing this reagent :

Yang L, Su T, Lv D, Xie F, Liu W, Cao J, et al. ERK1/2 mediates lung adenocarcinoma cell proliferation and autophagy induced by apelin-13. Acta Biochim Biophys Sin (Shanghai). 2014;46:100-11 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Cyclin D1 Antibody (monoclonal)

Catalog Number :

MA1033

Clonality :

Monoclonal (Clone: CY-D1)

Description short :

Mouse IgG monoclonal antibody for Cyclin D1, cyclin D1 (CCND1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;mouse;rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG2a

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, mouse, rat

Applications :

WB,IHC-P,IHC-F,ICC

Western blot :

At 4μg/ml with the appropriate system to detect cyclin D1 in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 8μg/ml to detect cyclin D1 in formalin fixed and paraffin embedded tissues.

Immunohistochemistry(Frozen Section) :

At 8μg/ml to detect cyclin D1 in formalin or acetone fixed tissues.

Immunocytochemistry :

Suitable

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Recombinant human cyclin D1 protein.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P), IHC(F) and ICC.

Background :

D-type cyclins(cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. CyclinD1(CCND1), also known as BCL1. Expression of cyclin D1, but not of cyclins A and E, was induced by transfection of the RB gene into RB-deficient tumor cells. CCND1 gene to 11q13. cyclin D1 gene can function as an oncogene. Cyclin D1 expression is regulated by the retinoblastoma protein.

Reference :

1. Rosenberg, C. L.; Kim, H. G.; Shows, T. B.; Kronenberg, H. M.; Arnold, A. : Rearrangement and overexpression of D11S287E, a candidate oncogene on chromosome 11q13 in benign parathyroid tumors. Oncogene 6: 449-453, 1991. 2. Muller, H.; Lukas, J.; Schneider, A.; Warthoe, P.; Bartek, J.; Eilers, M.; Strauss, M. : Cyclin D1 expression is regulated by the retinoblastoma protein. Proc. Nat. Acad. Sci. 91: 2945-2949, 1994. 3. Hinds, P. W.; Dowdy, S. F.; Eaton, E. N.; Arnold, A.; Weinberg, R. A. : Function of a human cyclin gene as an oncogene. Proc. Nat. Acad. Sci. 91: 709-713, 1994.

Gene Name :

CCND1

Protein Name :

G1/S-specific cyclin-D1

Gene Full Name :

cyclin D1

Synonyms :

BCL1 U21B31 D11S287 PRAD1 B-CELL LEUKEMIA 1 B-cell CLL/lymphoma 1 G1/S-specific cyclin D1 parathyroid adenomatosis 1 B-cell lymphoma 1 protein BCL-1 oncogene PRAD1 oncogene

Uniprot ID :

Human(P24385), Mouse(P25322), Rat(P39948)

more info or order :

Boster Immunoleader product webpage