Anti-Collagen, Type III Antibody (monoclonal) | Boster Immunoleader MA1029 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Collagen, Type III Antibody (monoclonal)

catalog :

MA1029

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - frozen section

publications citing this reagent :

Ge W, Wu J, Fan J, Wang Y, Chen Y. Inhibition of high-mobility group box 1 expression by siRNA in rat hepatic stellate cells. World J Gastroenterol. 2011;17:4090-8 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Collagen, Type III Antibody (monoclonal)

Catalog Number :

MA1029

Clonality :

Monoclonal (Clone: Col-29)

Description short :

Mouse IgG monoclonal antibody for Collagen, Type III, collagen, type III, alpha 1 (COL3A1) detection. Tested with WB, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, rat

Applications :

WB,IHC-F

Western blot :

At 0.25-0.5μg/ml with the appropriate system to detect collagen type III in cells and tissues.

Immunohistochemistry(Frozen Section) :

At 0.5-1μg/ml to detect collagen type III in formalin/acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Human collagen type III.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(F).

Background :

Collagen type III, also known as COL3A1, is assigned to chromosome 2. Ultrastructural analysis of tissues from mutant mice revealed that type III collagen is essential for normal collagen I fibrillogenesis in the cardiovascular system and other organs. Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development.

Reference :

1. Cutting, G. R.; McGinniss, M. J.; Kasch, I. M.; Tsipouras, P.; Antonarakis, S. E. : Physical mapping by PFGE localizes the COL3A1 and COL5A2 genes to a 35 kb region on chromosome 2. (Abstract) Clin. Res. 38: 266A, 1990. 2. Emanuel, B. S.; Cannizzaro, L. A.; Seyer, J. M.; Myers, J. C. : Human alpha-1(III) and alpha-2(V) procollagen genes are located on the long arm of chromosome 2. Proc. Nat. Acad. Sci. 82: 3385-3389, 1985. 3. Liu, X.; Wu, H.; Byrne, M.; Krane, S.; Jaenisch, R. : Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development. Proc. Nat. Acad. Sci. 94: 1852-1856, 1997.

Gene Name :

COL3A1

Protein Name :

Collagen alpha-1(III) chain

Gene Full Name :

collagen, type III, alpha 1

Synonyms :

EDS4A COLLAGEN, FETAL COLLAGEN, BLOOD VESSEL

Uniprot ID :

Human(P02461), Mouse(P08121), Rat(P13941)

more info or order :

Boster Immunoleader product webpage