Anti-c-Myc Antibody (monoclonal) | Boster Immunoleader MA1028 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-c-Myc Antibody (monoclonal)

catalog :

MA1028

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-c-Myc Antibody (monoclonal)

Catalog Number :

MA1028

Clonality :

Monoclonal (Clone: IMD-3)

Description short :

Mouse IgG monoclonal antibody for c-Myc, v-myc myelocytomatosis viral oncogene homolog (avian) (MYC) detection. Tested with WB, IHC-P, ICC in Human. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human

Applications :

WB,IHC-P,ICC

Western blot :

At 4μg/ml with the appropriate system to detect c-Myc in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 8μg/ml to detect c-Myc in formalin fixed and paraffin embedded tissues.

Immunocytochemistry :

Suitable

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and ICC.

Background :

C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol(2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.

Reference :

1. Liao D, Thakur A, Wu J, Biliran H, Sarkar FH. Perspectives on c-Myc, Cyclin D1, and Their Interaction in Cancer Formation, Progression, and Response to Chemotherapy.Crit Rev Oncog. 2007;13(2):93-158. 2. Britton S, Salles B, Calsou P. c-MYC protein is degraded in response to UV irradiation.Cell Cycle. 2007 Oct 2;7(1) 3. Chow JM, Liu CR, Lin CP, Lee CN, Cheng YC, Lin S, Liu HE. Downregulation of c-Myc determines sensitivity to 2-methoxyestradiol-induced apoptosis in human acute myeloid leukemia.Exp Hematol. 2008 Feb;36(2):140-8. 4. Huang CY, Bredemeyer AL, Walker LM, Bassing CH, Sleckman BP. Dynamic regulation of c-Myc proto-oncogene expression during lymphocyte development revealed by a GFP-c-Myc knock-in mouse.Eur J Immunol. 2008 Jan 14.

Gene Name :

MYC

Protein Name :

Myc proto-oncogene protein

Gene Full Name :

v-myc myelocytomatosis viral oncogene homolog(avian)

Synonyms :

C-Myc bHLHe39 Proto-oncogene c-Myc Transcription factor p64 Class E basic helix-loop-helix protein 39

Uniprot ID :

Human(P01106), Mouse(P01108), Rat(P09416)

more info or order :

Boster Immunoleader product webpage