Anti-Cytokeratin Peptide 18 Antibody (monoclonal) | Boster Immunoleader MA1026 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Cytokeratin Peptide 18 Antibody (monoclonal)

catalog :

MA1026

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Cytokeratin Peptide 18 Antibody (monoclonal)

Catalog Number :

MA1026

Clonality :

Monoclonal (Clone: CK-18)

Description short :

Mouse IgG monoclonal antibody for Cytokeratin Peptide 18, keratin 18 (KRT18) detection. Tested with WB, IHC-P, IHC-F in Human; rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, rat

Applications :

WB,IHC-P,IHC-F

Western blot :

At 0.5-1μg/ml with the appropriate system to detect cytokeratin peptide 18 in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 1-2μg/ml to detect cytokeratin peptide 18 in formalin fixed and paraffin embedded tissues.

Immunohistochemistry(Frozen Section) :

At 0.5-2μg/ml to detect cytokeratin peptide 18 in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and IHC(F).

Background :

Intermediate filaments(IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18(KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis.

Reference :

1. Ku, N.-O.; Wright, T. L.; Terrault, N. A.; Gish, R.; Omary, M. B. : Mutation of human keratin 18 in association with cryptogenic cirrhosis. J. Clin. Invest. 99: 19-23, 1997. 2. Waseem, A.; Gough, A. C.; Spurr, N. K.; Lane, E. B. : Localization of the gene for human simple epithelial keratin 18 to chromosome 12 using polymerase chain reaction. Genomics 7: 188-194, 1990.

Gene Name :

KRT18

Protein Name :

Keratin, type I cytoskeletal 18

Gene Full Name :

keratin 18

Synonyms :

K18 Keratin-18 CK-18 Cytokeratin-18 CYK18 Cell proliferation-inducing gene 46 protein

Uniprot ID :

Human(P05783), Mouse(P05784), Rat(Q5BJY9)

more info or order :

Boster Immunoleader product webpage