Anti-Cytokeratin Peptide 7 Antibody (monoclonal) | Boster Immunoleader MA1024 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Cytokeratin Peptide 7 Antibody (monoclonal)

catalog :

MA1024

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

publications citing this reagent :

Huang F, Cao J, Liu Q, Zou Y, Li H, Yin T. MAPK/ERK signal pathway involved expression of COX-2 and VEGF by IL-1β induced in human endometriosis stromal cells in vitro. Int J Clin Exp Pathol. 2013;6:2129-36 pubmed
Jiang Z, Guo Y, Ren H, Zou Y, Fan M, Lv Y, et al. Tumor necrosis factor (TNF)-α upregulates progesterone receptor-A by activating the NF-κB signaling pathway in human decidua after labor onset. Placenta. 2012;33:1-7 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Cytokeratin Peptide 7 Antibody (monoclonal)

Catalog Number :

MA1024

Clonality :

Monoclonal (Clone: CK-7)

Description short :

Mouse IgG monoclonal antibody for Cytokeratin Peptide 7, keratin 7 (KRT7) detection. Tested with WB, IHC-P, IHC-F in Human. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human

Applications :

WB,IHC-P,IHC-F

Western blot :

At 1-2μg/ml with the appropriate system to detect cytokeratin peptide 7 in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 2-4μg/ml to detect cytokeratin peptide 7 in formalin fixed and paraffin embedded tissues.

Immunohistochemistry(Frozen Section) :

At 2-4μg/ml to detect cytokeratin peptide 7 in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Cytoskeletal preparation of the RT4 human bladder carcinoma cell line.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and IHC(F).

Background :

KRT7 is a type II keratin of simple nonkeratinizing epithelia. The deduced 489-amino acid protein has a calculated molecular mass of about 54 kD. K7 contains 4 central alpha-helical segments with heptad repeats of hydrophobic residues characteristic of a coiled-coil region. Within this domain, K7 shares 73% homology with epidermal K6B. KRT7 gene contains 9 exons and spans more than 15.6 kb. K7 gene is mapped to chromosome 12. Keratin 7 is expressed in a wide range of epithelial structures in humans.

Reference :

1. Rosenberg, M.; Fuchs, E.; Le Beau, M. M.; Eddy, R. L.; Shows, T. B. : Three epidermal and one simple epithelial type II keratin genes map to human chromosome 12. Cytogenet. Cell Genet. 57: 33-38, 1991. 2. Smith, F. J. D.; Porter, R. M.; Corden, L. D.; Lunny, D. P.; Lane, E. B.; McLean, W. H. I. : Cloning of human, murine, and marsupial keratin 7 and a survey of K7 expression in the mouse. Biochem. Biophys. Res. Commun. 297: 818-827, 2002.

Gene Name :

KRT7

Protein Name :

Keratin, type II cytoskeletal 7

Gene Full Name :

keratin 7

Synonyms :

SCL cytokeratin 7 K2C7 K7 keratin Keratin-7 55K type II cytoskeletal keratin, type II cytoskeletal 7 sarcolectin KERATIN, SIMPLE EPITHELIAL KERATIN, TYPE II, CYTOSKELETAL, 7 SARCOLECTIN Type-II keratin Kb7

Uniprot ID :

Human(P08729), Mouse(Q9DCV7), Rat(Q6IG12)

more info or order :

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