Anti-Cdk7/CAK Antibody (monoclonal) | Boster Immunoleader MA1022 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Cdk7/CAK Antibody (monoclonal)

catalog :

MA1022

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Cdk7/CAK Antibody (monoclonal)

Catalog Number :

MA1022

Clonality :

Monoclonal (Clone: IMD-26)

Description short :

Mouse IgG monoclonal antibody for Cdk7/CAK, cyclin-dependent kinase 7 (CDK7) detection. Tested with WB, IHC-F, ICC in Human. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG2b

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human

Applications :

WB,IHC-F,ICC

Western blot :

At 0.25μg/ml with the appropriate system to detect cdk7/cak in cells and tissues.

Immunohistochemistry(Frozen Section) :

At 0.5μg/ml to detect cdk7/cak in formalin/acetone fixed tissues.

Immunocytochemistry :

Suitable

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Recombinant human Cdk7 protein.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(F) and ICC.

Background :

CDK-activating kinases(CAKs) are multisubunit proteins that phosphorylate and thus activate certain cyclin-dependent protein kinases in the regulation of cell cycle progression. Cyclin dependent kinase7(CDK7) gene is mapped to chromosome 2p15-cen. CDK7 functions in both cyclin binding and T-loop phosphorylation and that these 2 steps of CDK1 activation are mutually dependent.

Reference :

1. Levedakou, E. N.; He, M.; Baptist, E. W.; Craven, R. J.; Cance, W. G.; Welcsh, P. L.; Simmons, A.; Naylor, S. L.; Leach, R. L.; Lewis, T. B.; Bowcock, A.; Liu, E. T. : Two novel human serine/threonine kinases with homologies to the cell cycle regulating Xenopus MO15, and NIMA kinases: cloning and characterization of their expression pattern. Oncogene 9: 1977-1988, 1994.

Gene Name :

CDK7

Protein Name :

Cyclin-dependent kinase 7

Gene Full Name :

cyclin-dependent kinase 7

Synonyms :

CAK CDKN7 MO15 CAK1 STK1 p39 Mo15 KINASE SUBUNIT OF CAK SERINE/THREONINE PROTEIN KINASE 1 CELL DIVISION PROTEIN KINASE 7 39 kDa protein kinase CDK-activating kinase 1 Cell division protein kinase 7 Serine/threonine-protein kinase 1 TFIIH basal transcription factor complex kinase subunit

Uniprot ID :

Human(P50613), Mouse(Q03147), Rat(P51952)

more info or order :

Boster Immunoleader product webpage