Anti-CDC6 Antibody (monoclonal) | Boster Immunoleader MA1019 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-CDC6 Antibody (monoclonal)

catalog :

MA1019

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-CDC6 Antibody (monoclonal)

Catalog Number :

MA1019

Clonality :

Monoclonal (Clone: IMD-6)

Description short :

Mouse IgG monoclonal antibody for CDC6, cell division cycle 6 homolog (S. cerevisiae) (CDC6) detection. Tested with WB in Human. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human

Applications :

Western blot :

At 1-2μg/ml with the appropriate system to detect cdc6 in cells and tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Recombinant human Cdc6.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB.

Background :

In yeasts, Cdc6(Saccharomyces cerevisiae) and Cdc18(Schizosaccharomyces pombe) associate with the origin recognition complex(ORC) proteins to render cells competent for DNA replication. Cdc6 is overexpressed in human cancers, where it has a critical regulatory role in addition to DNA replication. Yan et al.(1998) showed that Cdc6 is expressed selectively in proliferating but not quiescent mammalian cells, both in culture and within tissues in intact animals. During the transition from a growth-arrested to a proliferative state, transcription of mammalian Cdc6 is regulated by E2F proteins, as revealed by a functional analysis of the human Cdc6 promoter and by the ability of exogenously expressed E2F proteins to stimulate the endogenous Cdc6 gene. They conclude that expression of human Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells.

Reference :

1. Gonzalez, S.; Klatt, P.; Delgado, S.; Conde, E.; Lopez-Rios, F.; Sanchez-Cespedes, M.; Mendez, J.; Antequera, F.; Serrano, M. : Oncogenic activity of Cdc6 through repression of the INK4/ARF locus. Nature 440: 702-706, 2006. 2. Yan, Z.; DeGregori, J.; Shohet, R.; Leone, G.; Stillman, B.; Nevins, J. R.; Williams, R. S. : Cdc6 is regulated by E2F and is essential for DNA replication in mammalian cells. Proc. Nat. Acad. Sci. 95: 3603-3608, 1998.

Gene Name :

CDC6

Protein Name :

Cell division control protein 6 homolog

Gene Full Name :

cell division cycle 6 homolog(S. cerevisiae)

Synonyms :

CDC18L HsCdc18 p62(cdc6) CELL DIVISION CYCLE 18, S. POMBE, HOMOLOG-LIKE CDC6(cell division cycle 6, S. cerevisiae) homolog CELL CYCLE CONTROLLER CDC6 CDC6-related protein Cdc18-related protein

Uniprot ID :

Human(Q99741), Mouse(O89033)

more info or order :

Boster Immunoleader product webpage