Anti-CDC25C Antibody (monoclonal) | Boster Immunoleader MA1018 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-CDC25C Antibody (monoclonal)

catalog :

MA1018

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-CDC25C Antibody (monoclonal)

Catalog Number :

MA1018

Clonality :

Monoclonal (Clone: IMD-25)

Description short :

Mouse IgG monoclonal antibody for CDC25C, cell division cycle 25 homolog C (S. pombe) (CDC25C) detection. Tested with WB in Human. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human

Applications :

Western blot :

At 1-2μg/ml with the appropriate system to detect cdc25c in cells and tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Recombinant human Cdc25c.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB.

Background :

Cell division sycle(CDC25) gene product is a protein-tyrosine phosphatase, activates a partially purified p34(cdc2)/cyclin B complex. The cdc25 protein also shares homology with a protein phosphatase with activity against both tyrosine and serine(and thus probably threonine) phosphate residues. CDC25C is expressed predominantly in G2 phase in healer cells. The human gene encodes a protein with a predicted molecular mass of 53,000 daltons whose C-terminal domain shares about 37% sequence identity with the fission yeast cdc25 mitotic inducer. CDC25C gene is mapped to 5q31.

Reference :

1. Sartor, H.; Ehlert, F.; Grzeschik, K.-H.; Muller, R.; Adolph, S. : Assignment of two human cell cycle genes, CDC25C and CCNB1, to 5q31 and 5q12, respectively. Genomics 13: 911-912, 1992. 2. Taviaux, S. A.; Demaille, J. G. : Localization of human cell cycle regulatory genes CDC25C to 5q31 and WEE1 to 11p15.3-11p.15.1 by fluorescence in situ hybridization. Genomics 15: 194-196, 1993.

Gene Name :

CDC25C

Protein Name :

M-phase inducer phosphatase 3

Gene Full Name :

cell division cycle 25 homolog C(S. pombe)

Synonyms :

CDC25 PPP1R60 MITOSIS INDUCER CDC25 cell division cycle 25C protein phosphatase 1, regulatory subunit 60 Dual specificity phosphatase Cdc25C

Uniprot ID :

Human(P30307), Mouse(P48967), Rat(D4ADT2)

more info or order :

Boster Immunoleader product webpage