Anti-CD3 Antibody (monoclonal) | Boster Immunoleader MA1015 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-CD3 Antibody (monoclonal)

catalog :

MA1015

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

immunohistochemistry - frozen section

publications citing this reagent :

Zhang R, Zhou S, Li C, Wang X, Tang Y, Chen R, et al. C-reactive protein/oxidised low-density lipoprotein/β2-glycoprotein I complex promotes atherosclerosis in diabetic BALB/c mice via p38mitogen-activated protein kinase signal pathway. Lipids Health Dis. 2013;12:42 pubmed publisher
Liu Y, Cao Y, Ye J, Wang W, Song K, Wang X, et al. Immunomodulatory effects of proanthocyanidin A-1 derived in vitro from Rhododendron spiciferum. Fitoterapia. 2010;81:108-14 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-CD3 Antibody (monoclonal)

Catalog Number :

MA1015

Clonality :

Monoclonal (Clone: CA-3)

Description short :

Mouse IgG monoclonal antibody for CD3, CD3e molecule, epsilon (CD3-TCR complex) (CD3E ) detection. Tested with IHC-F in Human. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human

Applications :

IHC-F

Immunohistochemistry(Frozen Section) :

At 1μg/ml to detect CD3 in formalin or acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Human thymocytes followed by Sezary T cells.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by SA1021 in IHC(F).

Background :

The alpha-beta heterodimeric T-cell antigen receptor binds antigen in association with major histocompatibility complex proteins on host cell surfaces. These 2 disulfide-linked glycoproteins, TCRA and TCRB are associated on T-cell surfaces with a complex of proteins called CD3(formerly T3). Human CD3 consists of at least 4 proteins: gamma, delta, epsilon, and zeta. The CD3-gamma/CD3-delta gene pair is within 300 kb of the CD3-epsilon gene and therefore these genes form a tightly linked cluster on 11q23. The clustering may be significant in terms of their simultaneous activations during T-cell development.

Reference :

1. Krissansen, G. W.; Owen, M. J.; Verbi, W.; Crumpton, M. J. : Primary structure of the T3 gamma subunit of the T3/T cell antigen receptor complex deduced from cDNA sequences: evolution of the T3 gamma and delta subunits. EMBO J. 5: 1799-1808, 1986. 2. Tunnacliffe, A.; Buluwela, L.; Rabbitts, T. H. : Physical linkage of three CD3 genes on human chromosome 11. EMBO J. 6: 2953-2957, 1987.

Gene Name :

CD3E

Protein Name :

T-cell surface glycoprotein CD3 epsilon chain

Gene Full Name :

CD3e molecule, epsilon(CD3-TCR complex)

Synonyms :

T3E T-cell surface antigen T3/Leu-4 epsilon chain

Uniprot ID :

Human(P07766), Mouse(P22646), Rat(D4A5M2)

more info or order :

Boster Immunoleader product webpage