Anti-Cathepsin L Antibody (monoclonal) | Boster Immunoleader MA1014 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Cathepsin L Antibody (monoclonal)

catalog :

MA1014

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Cathepsin L Antibody (monoclonal)

Catalog Number :

MA1014

Clonality :

Monoclonal (Clone: CP-L14)

Description short :

Mouse IgG monoclonal antibody for Cathepsin L, cathepsin L1 (CTSL1) detection. Tested with WB, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, mouse, rat

Applications :

WB,IHC-F

Western blot :

At 2μg/ml with the appropriate system to detect cathepsin L in cells and tissues.

Immunohistochemistry(Frozen Section) :

At 4μg/ml to detect cathepsin L in formalin/acetone fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Procathepsin L isolated from the human lung cancer cell line EPLC 32M1.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(F).

Background :

Cathepsin L is a lysosomal cysteine proteinase with a major role in intracellular protein catabolism. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. Cathepsin L has been implicated in pathologic processes including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. Human liver cathepsin L consists of a heavy chain of about 25 kD and a light chain of about 5 kD. The gene is mapped to 9q21-q22. Cathepsin L is required for endothelial progenitor cell-induced neovascularization.

Reference :

1. Fan, Y.-S.; Byers, M. G.; Eddy, R. L.; Joseph, L.; Sukhatme, V.; Chan, S.-J.; Shows, T. B. : Cathepsin L (CTSL) is located in the chromosome 9q21-q22 region: a related sequence is located on chromosome 10. (Abstract) Cytogenet. Cell Genet. 51: 996 only, 1989. 2. Urbich, C.; Heeschen, C.; Aicher, A.; Sasaki, K.; Bruhl, T.; Farhadi, M. R.; Vajkoczy, P.; Hofmann, W. K.; Peters, C.; Pennacchio, L. A.; Abolmaali, N. D.; Chavakis, E.; Reinheckel, T.; Zeiher, A. M.; Dimmeler, S. : Cathepsin L is required for endothelial progenitor cell-induced neovascularization. Nature Med. 11: 206-213, 2005.

Gene Name :

CTSL1

Protein Name :

Cathepsin L1

Gene Full Name :

cathepsin L1

Synonyms :

CATL MEP CTSL cathepsin L Major excreted protein

Uniprot ID :

Human(P07711), Mouse(P06797), Rat(P07154)

more info or order :

Boster Immunoleader product webpage