Anti-Catenin gamma(Plakoglobin) Antibody (monoclonal) | Boster Immunoleader MA1012 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Catenin gamma(Plakoglobin) Antibody (monoclonal)

catalog :

MA1012

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat, cow

application :

western blot, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Catenin gamma(Plakoglobin) Antibody (monoclonal)

Catalog Number :

MA1012

Clonality :

Monoclonal (Clone: CN-3)

Description short :

Mouse IgG monoclonal antibody for Catenin gamma (Plakoglobin), junction plakoglobin ( JUP) detection. Tested with WB, IHC-F, ICC in Human;chicken;rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, bovine

Applications :

WB,IHC-F,ICC

Western blot :

At 2μg/ml with the appropriate system to detect plakoglobin in cells and tissues.

Immunohistochemistry(Frozen Section) :

At 4μg/ml to detect plakoglobin in formalin/acetone fixed tissues.

Immunocytochemistry :

Suitable

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Recombinant chicken plakoglobin.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(F) and ICC.

Background :

Junction Plakoglobin(JUP) ,also knows as catenin gamma, is a major cytoplasmic protein that occurs in a soluble and a membrane-associated form and is the only known constituent common to the submembranous plaques of both kinds of adhering junctions, the desmosomes and the intermediate junctions. It is a component of the cadherin-catenin complex, which is predominantly localized where actin filaments anchor in adherens junctions of epithelial cells. The human plakoglobin gene localizes on chromosome 17q21. Gamma-catenin is regulated by the APC tumor suppressor and its oncogenic activity is distinct from that of beta-catenin.

Reference :

1. Aberle, H.; Bierkamp, C.; Torchard, D.; Serova, O.; Wagner, T.; Natt, E.; Wirsching, J.; Heidkamper, C.; Montagna, M.; Lynch, H. T.; Lenoir, G. M.; Scherer, G.; Feunteun, J.; Kemler, R. : The human plakoglobin gene localizes on chromosome 17q21 and is subjected to loss of heterozygosity in breast and ovarian cancers. Proc. Nat. Acad. Sci. 92: 6384-6388, 1995. 2. Kolligs, F. T.; Kolligs, B.; Hajra, K. M.; Hu, G.; Tani, M.; Cho, K. R.; Fearon, E. R. : Gamma-catenin is regulated by the APC tumor suppressor and its oncogenic activity is distinct from that of beta-catenin. Genes Dev. 14: 1319-1331, 2000.

Gene Name :

JUP

Protein Name :

Junction plakoglobin

Gene Full Name :

junction plakoglobin

Synonyms :

DP3 PDGB PKGB CTNNG DPIII ARVD12 Catenin gamma Desmoplakin III Desmoplakin-3

Uniprot ID :

Human(P14923), Mouse(Q02257), Rat(Q6P0K8)

more info or order :

Boster Immunoleader product webpage