product summary
request information :
company name :
Boster Immunoleader
product type :
antibody
product name :
Anti-Calponin Antibody (monoclonal)
catalog :
MA1011
quantity :
100 ug/vial
price :
200 USD
clonality :
monoclonal
host :
mouse
reactivity :
human, mouse, chicken
application :
western blot, immunocytochemistry, immunohistochemistry - paraffin section
product information
Product Name :
Anti-Calponin Antibody (monoclonal)
Catalog Number :
MA1011
Clonality :
Monoclonal (Clone: CP-19)
Description short :
Mouse IgG monoclonal antibody for Calponin, calponin 2 (CNN2) detection. Tested with WB, IHC-P, ICC in Human;mouse;rat;chicken. No cross reactivity with other proteins.
Price :
200 USD
Ig type :
Mouse IgG1
Size :
100 ug/vial
Form :
Lyophilized
Specificity :
No cross reactivity with other proteins.
Reactivity :
Human, chicken, turkey
Applications :
WB,IHC-P,ICC
Western blot :
At 0.25-0.5μg/ml with the appropriate system to detect calponin in cells and tissues.
Immunohistochemistry(Paraffin-embedded S ... :
At 0.5-1μg/ml to detect calponin in formalin fixed and paraffin embedded tissues.
Immunocytochemistry :
Suitable
Notes :
Other applications have not been tested. Optimal dilutions should be determined by end users.
Immunogen :
Turkey gizzard calponin
Contents :
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Purification :
Ascites
Reconstitution :
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
Storage :
At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.
Relevant detection systems :
Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P) and ICC.
Background :
Calponin is a basic 34-kD protein first isolated from chicken gizzard and bovine aorta. It is specifically expressed in smooth muscle and binds calmodulin, actin, and tropomyosin. It is able to inhibit the ATPase activity of myosin and is though to play a role in smooth muscle contraction. Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein though to be involved in some way in the regulation or modulation of contraction.
Reference :
1. Strasser, P.; Gimona, M.; Moessler, H.; Herzog, M.; Small, J. V. : Mammalian calponin: identification and expression of genetic variants. FEBS Lett. 330: 13-18, 1993.
Gene Name :
CNN2
Protein Name :
Calponin-2
Gene Full Name :
calponin 2
Synonyms :
Calponin H2, smooth muscle Neutral calponin
Uniprot ID :
Human(Q99439), Mouse(Q08093)
company information
Boster Immunoleader
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
www.bosterbio.com
925.485.4527
headquarters: USA
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