Anti-BIN1 Antibody (monoclonal) | Boster Immunoleader MA1005 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-BIN1 Antibody (monoclonal)

catalog :

MA1005

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-BIN1 Antibody (monoclonal)

Catalog Number :

MA1005

Clonality :

Monoclonal (Clone: BN-1)

Description short :

Mouse IgG monoclonal antibody for BIN1, bridging integrator 1 (BIN1) detection. Tested with WB, IHC-F, ICC in Human;mouse;rat. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG2b

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, mouse, rat

Applications :

WB,IHC-F,ICC

Western blot :

At 0.25μg/ml with the appropriate system to detect Bin1 in cells and tissues.

Immunohistochemistry(Frozen Section) :

At 0.5μg/ml to detect Bin1in formalin/acetone fixed tissues.

Immunocytochemistry :

Suitable

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Recombinant polypeptide containing amino acids 189-398 of human Bin1.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(F) and ICC.

Background :

BIN1(AMPH2) is a novel human gene product with features of a tumor suppressor protein. BIN1 gene to chromosome 2q14. Loss of BIN1 expression appears to be a frequent aberration in human hepatocellular carcinomas . mutations in BIN1 cause centronuclear myopathy by interfering with remodeling of T tubules and/or endocytic membranes, and that the functional interaction between BIN1 and DNM2 is necessary for normal muscle function and positioning of nuclei.

Reference :

1. Sakamuro, D.; Elliott, K. J.; Wechsler-Reya, R.; Prendergast, G. C. : BIN1 is a novel MYC-interacting protein with features of a tumour suppressor. Nature Genet. 14: 69-77, 1996. 2. Negorev, D.; Riethman, H.; Wechsler-Reya, R.; Sakamuro, D.; Prendergast, G. C.; Simon, D. : The Bin1 gene localizes to human chromosome 2q14 by PCR analysis of somatic cell hybrids and fluorescence in situ hybridization. Genomics 33: 329-331, 1996. 3. Nicot, A.-S.; Toussaiant, A.; Tosch, V.; Kretz, C.; Wallgren-Petterson, C.; Iwarsson, E.; Kingston, H.; Garnier, J.-M.; Biancalana, V.; Oldfors, A.; Mandel, J.-L.; Laporte, J. : Mutations in amphiphysin 2 (BIN1) disrupt interaction with dynamin 2 and cause autosomal recessive centronuclear myopathy. (Letter) Nature Genet. 39: 1134-1139, 2007.

Gene Name :

BIN1

Protein Name :

Myc box-dependent-interacting protein 1

Gene Full Name :

bridging integrator 1

Synonyms :

AMPHL AMPH2 SH3P9 amphiphysin II Amphiphysin-like protein;Box-dependent myc-interacting protein 1;Bridging integrator 1

Uniprot ID :

Human(O00499), Mouse(O08539), Rat(O08839)

more info or order :

Boster Immunoleader product webpage