Anti-Bcl-2 Antibody (monoclonal) | Boster Immunoleader MA1004 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Bcl-2 Antibody (monoclonal)

catalog :

MA1004

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

publications citing this reagent :

Zhen-Qiang F, Bing-Wei Y, Yong-Liang L, Xiang-Wei W, Shan-Hong Y, Yuan-Ning Z, et al. Localized expression of human BMP-7 by BM-MSCs enhances renal repair in an in vivo model of ischemia-reperfusion injury. Genes Cells. 2012;17:53-64 pubmed publisher
Yang J, Li J, Lu J, Zhang Y, Zhu Z, Wan H. Synergistic protective effect of astragaloside IV-tetramethylpyrazine against cerebral ischemic-reperfusion injury induced by transient focal ischemia. J Ethnopharmacol. 2012;140:64-72 pubmed publisher
Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, et al. Phytoestrogen calycosin-7-O-β-D-glucopyranoside ameliorates advanced glycation end products-induced HUVEC damage. J Cell Biochem. 2011;112:2953-65 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Bcl-2 Antibody (monoclonal)

Catalog Number :

MA1004

Clonality :

Monoclonal (Clone: BL-2)

Description short :

Mouse IgG monoclonal antibody for Bcl-2, B-cell CLL/lymphoma 2 (BCL2) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG1

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human

Applications :

WB,IHC-P,IHC-F,ICC

Western blot :

At 1-2μg/ml with the appropriate system to detect BCL-2 in cells and tissues.

Immunohistochemistry(Paraffin-embedded S ... :

At 0.4-1μg/ml to detect BCL-2 in formalin fixed and paraffin embedded tissues.

Immunohistochemistry(Frozen Section) :

At 0.4-1μg/ml to detect BCL-2 in formalin/acetone fixed tissues.

Immunocytochemistry :

Suitable

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Synthetic peptide corresponding to residues 41-54 of the bcl-2 protein, conjugated to thyroglobulin.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(P), IHC(F) and ICC.

Background :

Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.

Reference :

1. Ngan, B.-Y.; Chen-Levy, Z.; Weiss, L. M.; Warnke, R. A.; Cleary, M. L. : Expression in non-Hodgkin's lymphoma of the BCL-2 protein associated with the t(14;18) chromosomal translocation. New Eng. J. Med. 318: 1638-1644, 1988. 2. Hockenbery, D.; Nunez, G.; Milliman, C.; Schreiber, R. D.; Korsmeyer, S. J. : Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348: 334-336, 1990.

Gene Name :

BCL2

Protein Name :

Apoptosis regulator Bcl-2

Gene Full Name :

B-cell CLL/lymphoma 2

Synonyms :

PPP1R50 protein phosphatase 1, regulatory subunit 50 ONCOGENE B-CELL LEUKEMIA 2

Uniprot ID :

Human(P10415), Mouse(P10417), Rat(P49950)

more info or order :

Boster Immunoleader product webpage