Anti-Actin Antibody (monoclonal) | Boster Immunoleader MA1000 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Actin Antibody (monoclonal)

catalog :

MA1000

quantity :

100 ug/vial

price :

200 USD

clonality :

monoclonal

host :

mouse

application :

western blot, immunohistochemistry - frozen section

publications citing this reagent :

Yang L, Liu J, Bai X, Fan L, Han F, Jia W, et al. Acute downregulation of miR-155 at wound sites leads to a reduced fibrosis through attenuating inflammatory response. Biochem Biophys Res Commun. 2014;453:153-9 pubmed publisher
Xu H, Wei W, Jia X, Chang Y, Zhang L. Effects and mechanisms of total glucosides of paeony on adjuvant arthritis in rats. J Ethnopharmacol. 2007;109:442-8 pubmed

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Actin Antibody (monoclonal)

Catalog Number :

MA1000

Clonality :

Monoclonal (Clone: AC-40)

Description short :

Mouse IgG monoclonal antibody for Actin detection. Tested with WB, IHC-F, ICC in Human;mouse;rat;chicken. No cross reactivity with other proteins.

Price :

200 USD

Ig type :

Mouse IgG2a

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Human, chicken, mouse, rat

Applications :

WB,IHC-F

Western blot :

At 2μg/ml with the appropriate system to detect Actin in cells and tissues.

Immunohistochemistry(Frozen Section) :

At 4μg/ml to detect Actin in methanol-fixed tissues.

Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

Synthetic actin C-terminal peptideSer-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe,attached to a Multiple Antigen Peptide(MAP) backbone.

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Reconstitution :

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1001 in WB, supported by SA1021 in IHC(F).

Background :

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Reference :

1. Gunning,P., Ponte,P., Okayama,H., Engel,J., Blau,H. and Kedes,L.Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed. Mol. Cell. Biol.1983; 3 (5), 787-795. 2. Goebel,H.H., Brockmann,K., Bonnemann,C.G., Warlo,I.A., Hanefeld,F.,Labeit,S., Durling,H.J. and Laing,N.G.Actin-related myopathy without any missense mutation in the ACTA1 Gene. J. Child Neurol.2004; 19 (2), 149-153. 3. Laing,N.G., Clarke,N.F., Dye,D.E., Liyanage,K., Walker,K.R.,Kobayashi,Y., Shimakawa,S., Hagiwara,T., Ouvrier,R., Sparrow,J.C., Nishino,I., North,K.N. and Nonaka,I.Actin mutations are one cause of congenital fibre type disproportion.Ann. Neurol.2004; 56 (5), 689-694.

Uniprot ID :

Human(P68133), Mouse(P68134), Rat(P68136), Human(P68032), Mouse(P68033), Rat(P68035), Human(P62736), Mouse(P68134), Rat(P68136), Human(P60709), Mouse(P60710), Rat(P60711), Human(P63261), Mouse(P63260), Rat(P63269), Human(P63267), Mouse(P63268), Rat(P63269)

more info or order :

Boster Immunoleader product webpage