Boster Immunoleader

ELISA/assay

Human CD73/NT5E PicoKine ELISA Kit

EK1422

1 x96 well plate

369 USD

Human CD73/NT5E PicoKine ELISA Kit

EK1422

369 USD

Sandwich ELISA kit for Quantitative Detection of Human CD73/NT5E

CHO,W27-K547

1 x96 well plate

31.2pg/ml-2000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 132; Standard deviation: 5.94; CV(%): 4.5 Sample 2: n=16; Mean(pg/ml): 510; Standard deviation: 28.56; CV(%): 5.6 Sample 3: n=16; Mean(pg/ml): 1427; Standard deviation: 91.33; CV(%): 6.4

Sample 1: n=24; Mean(pg/ml): 167; Standard deviation: 8.85; CV(%): 5.3 Sample 2: n=24; Mean(pg/ml): 624; Standard deviation: 37.44; CV(%): 6 Sample 3: n=24; Mean(pg/ml): 1553; Standard deviation: 111.82; CV(%): 7.2

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human CD73 in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human CD73 antibody 1 ST0000-10 Lyophilized recombinant human CD73 standard 10ng/tube×2 AR1107 Biotinylated anti- human CD73 antibody 130μl(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

NT5E

5'-nucleotidase

5'-nucleotidase, ecto (CD73)

5' NT 5' nucleotidase (CD73) 5' nucleotidase precursor 5' nucleotidase, ecto 5' nucleotidase, ecto (CD73) 5'-NT 5'-nucleotidase 5NTD_HUMAN CD73 CD73 antigen E5NT Ecto 5' nucleotidase Ecto-5'-nucleotidase eN eNT NT NT5 NT5E NTE Purine 5 Prime Nucleotidase

P21589