Boster Immunoleader

ELISA/assay

Human COMT PicoKine ELISA Kit

EK1406

1 x96 well plate

369 USD

Human COMT PicoKine ELISA Kit

EK1406

369 USD

Sandwich ELISA kit for Quantitative Detection of Human COMT

E.coli, G52-P271

1 x96 well plate

31.2pg/ml-2000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 305; Standard deviation: 15.55; CV(%): 5.1 Sample 2: n=16; Mean(pg/ml): 818; Standard deviation: 43.35; CV(%): 5.3 Sample 3: n=16; Mean(pg/ml): 1249; Standard deviation: 77.43; CV(%): 6.2

Sample 1: n=24; Mean(pg/ml): 254; Standard deviation: 16.25; CV(%): 6.4 Sample 2: n=24; Mean(pg/ml): 699; Standard deviation: 38.44; CV(%): 5.5 Sample 3: n=24; Mean(pg/ml): 1358; Standard deviation: 92.34; CV(%): 6.8

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human COMT in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human COMT antibody 1 ST0000-10 Lyophilized recombinant human COMT standard 10ng/tube×2 AR1107 Biotinylated anti- human COMT antibody 130μl(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Catechol-O-methyltransferase (COMT) is one of several enzymes that degrade catecholamines such as dopamine, epinephrine, and norepinephrine. In humans, catechol-O-methyltransferase protein is encoded by the COMT gene. As the regulation of catecholamines is impaired in a number of medical conditions, several pharmaceutical drugs target COMT to alter its activity and therefore the availability of catecholamines. In the brain, COMT-dependent dopamine degradation is of particular importance in brain regions with low expression of the presynaptic dopamine transporter (DAT), such as the prefrontal cortex. This process is supposed to take place in postsynaptic neurons, as, in general, COMT is located intracellularly in the central nervous system (CNS).

COMT

Catechol O-methyltransferase

catechol-O-methyltransferase

Catechol O methyltransferase Catechol O-methyltransferase COMT COMT_HUMAN EC 2.1.1.6

P21964