Boster Immunoleader

ELISA/assay

Human Stanniocalcin 1/STC1 PicoKine ELISA Kit

EK1404

1 x96 well plate

333 USD

Human Stanniocalcin 1/STC1 PicoKine ELISA Kit

EK1404

333 USD

Sandwich ELISA kit for Quantitative Detection of Human Stanniocalcin 1/STC1

NSO, V34-A247

1 x96 well plate

31.2pg/ml-2000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 246; Standard deviation: 14.02; CV(%): 5.7 Sample 2: n=16; Mean(pg/ml): 801; Standard deviation: 48.06; CV(%): 6.0 Sample 3: n=16; Mean(pg/ml): 1351; Standard deviation: 74.30; CV(%): 5.5

Sample 1: n=24; Mean(pg/ml): 254; Standard deviation: 15.74; CV(%): 6.2 Sample 2: n=24; Mean(pg/ml): 775; Standard deviation: 49.6; CV(%): 6.4 Sample 3: n=24; Mean(pg/ml): 1416; Standard deviation: 67.96; CV(%): 6.8

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human STC1 in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human STC1 antibody 1 ST0000-10 Lyophilized recombinant human STC1 standard 10ng/tube×2 AR1107 Biotinylated anti- human STC1 antibody 130μl(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Stanniocalcin-1 is a glycoprotein which is encoded by the STC1 gene. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. Human Stanniocalcin-1 is a putative molecular biomarker of leukemic microenvironment and the only molecular function known up to date is a SUMO E3 ligase activity in the SUMOylation cycle. STC1 interacts with lots of proteins in the cytoplasm, mitochondria, endoplasmatic reticulum and dot-like fashion in the nucleus. The N-terminal region of STC1 is the function region which is responsible to establish the interaction with its partners, including SUMO1. Low resolution studies shows that STC1 is an anti-parallel homodimer in solution and the cystein 202 is responsible for the dimerization of this protein. All the 5 dissulfide bonds of human STC1 are conserved and have the same profile of fish STC.

STC1

Stanniocalcin-1

stanniocalcin 1

Stanniocalcin Stanniocalcin-1 STC STC-1 STC1 STC1_HUMAN

P52823