Boster Immunoleader

ELISA/assay

Human ERBB3/Her3 PicoKine ELISA Kit

EK1398

1 x96 well plate

399 USD

Human ERBB3/Her3 PicoKine ELISA Kit

EK1398

399 USD

Sandwich ELISA kit for Quantitative Detection of Human ERBB3/Her3

NSO, S20-T643

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 1.65; Standard deviation: 0.086; CV(%): 5.2 Sample 2: n=16; Mean(ng/ml): 4.4; Standard deviation: 0.198; CV(%): 4.5 Sample 3: n=16; Mean(ng/ml): 6.25; Standard deviation: 0.294; CV(%): 4.7

Sample 1: n=24; Mean(ng/ml): 1.37; Standard deviation: 0.081; CV(%): 5.9 Sample 2: n=24; Mean(ng/ml): 3.56; Standard deviation: 0.182; CV(%): 5.1 Sample 3: n=24; Mean(ng/ml): 7.19; Standard deviation: 0.446; CV(%): 6.2

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human ERBB3 in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human ERBB3 antibody 1 ST0000-10 Lyophilized recombinant human ERBB3 standard 10ng/tube×2 AR1107 Biotinylated anti- human ERBB3 antibody 130μl(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3), is a membrane bound protein that in humans is encoded by the ERBB3 gene. ErbB3 has been shown to bind the ligands heregulin and NRG-2. Ligand binding causes a change in conformation that allows for dimerization, phosphorylation, and activation of signal transduction. ErbB3 can heterodimerize with any of the other three ErbB family members. The theoretical ErbB3 homodimer would be non-functional because the kinase-impaired protein requires transphosporylation by its binding partner to be active. Unlike the other ErbB receptor tyrosine kinase family members which are activated through autophosphorylation upon ligand binding, ErbB3 is found to be kinase impaired, having only 1/1000th the autophosphorylation activity of EGFR and no ability to phosphorylate other proteins. Therefore, ErbB3 must act as anallosteric activator.

ERBB3

Receptor tyrosine-protein kinase erbB-3

erb-b2 receptor tyrosine kinase 3

c erbB 3 c erbB3 Erb b2 receptor tyrosine kinase 3 ErbB 3 ERBB3 ERBB3 protein erbB3 S ERBB3_HUMAN Glial growth factor receptor HER 3 HER3 Human epidermal growth factor receptor 3 LCCS2 MDA BF 1 MGC88033 p180 ErbB3 p45 sErbB3 p85 sErbB3 proto-oncogene-like protein c ErbB 3 proto-oncogene-like protein c ErbB3 Proto-oncogene-like protein c-ErbB-3 Receptor tyrosine protein kinase erbB 3 Receptor tyrosine protein kinase erbB3 Receptor tyrosine-protein kinase erbB-3 Tyrosine kinase type cell surface receptor HER3 Tyrosine kinase-type cell surface receptor HER3 v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) v erb b2 erythroblastic leukemia viral oncogene homolog 3

P21860