Boster Immunoleader

ELISA/assay

Mouse FGF21 PicoKine ELISA Kit

EK1379

1 x96 well plate

333 USD

Mouse FGF21 PicoKine ELISA Kit

EK1379

333 USD

Sandwich ELISA kit for Quantitative Detection of Mouse FGF21

E.coli, A29-S210

1 x96 well plate

31.2pg/ml-2000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 245; Standard deviation: 11.51; CV(%): 4.7 Sample 2: n=16; Mean(pg/ml): 656; Standard deviation: 28.20; CV(%): 4.3 Sample 3: n=16; Mean(pg/ml): 1422; Standard deviation: 71.1; CV(%): 5.0

Sample 1: n=24; Mean(pg/ml): 210; Standard deviation: 12.81; CV(%): 6.1 Sample 2: n=24; Mean(pg/ml): 670; Standard deviation: 43.55; CV(%): 6.5 Sample 3: n=24; Mean(pg/ml): 1572; Standard deviation: 99.03; CV(%): 6.3

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse FGF21 in cell culture supernates, serum and plasma(heparin, EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- Mouse FGF21 antibody 1 ST0000-10 Lyophilized recombinant Mouse FGF21 standard 10ng/tube×2 AR1107 Biotinylated anti- Mouse FGF21 antibody 130μl(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.

FGF21

Fibroblast growth factor 21

fibroblast growth factor 21

FGF 21 FGF-21 Fgf21 FGF21_HUMAN Fibroblast growth factor 21

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