Boster Immunoleader

ELISA/assay

Mouse Endostatin PicoKine ELISA Kit

EK1376

1 x96 well plate

369 USD

Mouse Endostatin PicoKine ELISA Kit

EK1376

369 USD

Sandwich ELISA kit for Quantitative Detection of Mouse Endostatin

NSO, H1591-K1774

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 0.8; Standard deviation: 0.052; CV(%): 6.5 Sample 2: n=16; Mean(ng/ml): 2.14; Standard deviation: 0.135; CV(%): 6.3 Sample 3: n=16; Mean(ng/ml): 4.25; Standard deviation: 0.179; CV(%): 4.2

Sample 1: n=24; Mean(ng/ml): 0.84; Standard deviation: 0.06; CV(%): 7.1 Sample 2: n=24; Mean(ng/ml): 2.23; Standard deviation: 0.147; CV(%): 6.6 Sample 3: n=24; Mean(ng/ml): 4.37; Standard deviation: 0.253; CV(%): 5.8

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse Endostatin in cell culture supernates, serum and plasma(heparin, EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse Endostatin antibody 1 ST0000-10 Lyophilized mouse Endostatin standard 10ng/tube×2 AR1107 Biotinylated anti- mouse Endostatin antibody 130μl(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.

COL18A1

Collagen alpha-1(XVIII) chain

collagen, type XVIII, alpha 1

Alpha 1 collagen type 18 (XVIII)(COL18A1) Alpha 1 type XVIII collagen Antiangiogenic agent COL18A1 Collagen alpha 1(XVIII) chain Collagen type XVIII alpha 1 FLJ27325 FLJ34914 KNO KNO1 MGC74745 Multi functional protein MFP OTTMUSP00000023764 OTTMUSP00000023766 Procollagen type XVIII alpha 1

P39061