Boster Immunoleader

ELISA/assay

Human Biglycan PicoKine ELISA Kit

EK1357

1 x96 well plate

369 USD

Human Biglycan PicoKine ELISA Kit

EK1357

369 USD

Sandwich ELISA kit for Quantitative Detection of Human Biglycan

NSO, D38-K368

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 1.62; Standard deviation: 0.06; CV(%): 3.7 Sample 2: n=16; Mean(ng/ml): 2.91; Standard deviation: 0.157; CV(%): 5.4 Sample 3: n=16; Mean(ng/ml): 5.38; Standard deviation: 0.264; CV(%): 4.9

Sample 1: n=24; Mean(ng/ml): 1.52; Standard deviation: 0.062; CV(%): 4.1 Sample 2: n=24; Mean(ng/ml): 3.07; Standard deviation: 0.209; CV(%): 6.8 Sample 3: n=24; Mean(ng/ml): 6.34; Standard deviation: 0.33; CV(%): 5.2

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human Biglycan in cell culture supernates, serum, plasma(heparin, EDTA) and tissue homogenates.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human Biglycan antibody 1 ST0000-10 Lyophilized recombinant human Biglycan standard 10ng/tube×2 AR1107 Biotinylated anti- human Biglycan antibody 130μl(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Biglycan (BGN) is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. It is an important structural component of articular cartilage and participates in the assembly of the chondrocyte extracellular matrix through formation of protein interactions with type VI collagen and large proteoglycan aggregates. In humans, Biglycan is encoded by the BGN gene. It is mapped to Xq28. Biglycan is believed to play a role in the mineralisation of bone, and its core protein binds to the growth factors BMP-4 and influences its bioactivity. It has been reported that the presence of biglycan is necessary for BMP-4 to exert its effects on osteoblasts. There is also evidence that biglycan can bind to TGF-beta 1. In addition, Biglycan plays an important role in cell signaling.

BGN

Biglycan

biglycan

BGN Biglycan Biglycan proteoglycan Bone/cartilage proteoglycan I Dermatan sulphate proteoglycan I DSPG1 PG S1 PG-S1 PGI PGS1_HUMAN SLRR1A Small leucine rich protein 1A

P21810