Boster Immunoleader

ELISA/assay

Human Neuropilin-1 PicoKine ELISA Kit

EK1353

1 x96 well plate

399 USD

Human Neuropilin-1 PicoKine ELISA Kit

EK1353

399 USD

Sandwich ELISA kit for Quantitative Detection of Human Neuropilin-1

NSO, F22-K644

1 x96 well plate

0.78ng/ml-50ng/ml

<20pg/ml

Sample 1: n=16; Mean(ng/ml): 6.8; Standard deviation: 0.374; CV(%): 5.5 Sample 2: n=16; Mean(ng/ml): 16; Standard deviation: 0.752; CV(%): 4.7 Sample 3: n=16; Mean(ng/ml): 25; Standard deviation: 0.85; CV(%): 3.4

Sample 1: n=24; Mean(ng/ml): 7.3; Standard deviation: 0.445; CV(%): 6.1 Sample 2: n=24; Mean(ng/ml): 20; Standard deviation: 1.04; CV(%): 5.2 Sample 3: n=24; Mean(ng/ml): 31; Standard deviation: 1.49; CV(%): 4.8

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human Neuropilin-1 in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human Neuropilin-1 antibody 1 ST0000-50 Lyophilized recombinant human Neuropilin-1 standard 50ng/tube×2 AR1107 Biotinylated anti- human Neuropilin-1 antibody 130μl(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

NRP1(Neuropilin 1) also known as NP1, NRP, BDCA4 or VEGF165R, is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF) and semaphorin family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. By somatic cell hybrid analysis, the NRP1 gene was mapped to chromosome 10. NRP1 bounds PGF1 with lower affinity. NRP1-mediated interactions are a necessary element in the initiation of the primary immune response and offer another example, like that of agrin, of a molecule shared by neurologic and immunologic synapses. After T-cell contact with DC, T-cell NRP1 colocalized with CD3 in the immunologic synapse and, sometimes, also at the opposite pole of the T cell. Soluble NRP1 interacts in a homophilic fashion with NRP1 on both DC and T cells, and this binding can be inhibited by blocking antibodies to NRP1. Furthermore, selective NRP1 inhibition in this model suppressed neovascular formation substantially.

NRP1

Neuropilin-1

neuropilin 1

A5 protein BDCA4 BLOOD DENDRITIC CELL ANTIGEN 4 CD 304 CD304 DKFZp686A03134 DKFZp781F1414 Neuropilin-1 Neuropilin1 Neuropilin1 transmembrane receptor NP1 NPN1 NRP 1 NRP NRP1 NRP1_HUMAN Vascular endothelial cell growth factor 165 receptor VEGF165R

O14786