Boster Immunoleader

ELISA/assay

Mouse Osteoactivin/GPNMB PicoKine ELISA Kit

EK1331

1 x96 well plate

369 USD

Mouse Osteoactivin/GPNMB PicoKine ELISA Kit

EK1331

369 USD

Sandwich ELISA kit for Quantitative Detection of Mouse Osteoactivin/GPNMB

NSO, K23-N502

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 1174; Standard deviation: 64.57; CV(%): 5.5 Sample 2: n=16; Mean(pg/ml): 3248; Standard deviation: 207.9; CV(%): 6.4 Sample 3: n=16; Mean(pg/ml): 5532; Standard deviation: 271.1; CV(%): 4.9

Sample 1: n=24; Mean(pg/ml): 1354; Standard deviation: 82.6; CV(%): 6.1 Sample 2: n=24; Mean(pg/ml): 3426; Standard deviation: 246.7; CV(%): 7.2 Sample 3: n=24; Mean(pg/ml): 5840; Standard deviation: 315.4; CV(%): 5.4

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse Osteoactivin in cell culture supernates, serum and plasma(heparin, EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse Osteoactivin antibody 1 ST0000-10 Lyophilized recombinant mouse Osteoactivin standard 10ng/tubex2 AR1107 Biotinylated anti- mouse Osteoactivin antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Transmembrane glycoprotein NMB is a protein that is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis(soft callus) and osteogenesis(hard callus) compared to the intact femurs that is why Osteoactivin(OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.

GPNMB

Transmembrane glycoprotein NMB

glycoprotein (transmembrane) nmb

Gpnmb GPNMB_HUMAN Transmembrane glycoprotein HGFIN Transmembrane glycoprotein NMB

Q99P91