Boster Immunoleader

ELISA/assay

Rat IL-3 PicoKine ELISA Kit

EK1324

1 x96 well plate

333 USD

Rat IL-3 PicoKine ELISA Kit

EK1324

333 USD

Sandwich ELISA kit for Quantitative Detection of Rat IL-3

E.coli, I27-C169

1 x96 well plate

7.8pg/ml-500pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 44; Standard deviation: 2.29; CV(%): 5.2 Sample 2: n=16; Mean(pg/ml): 166; Standard deviation: 11.8; CV(%): 7.1 Sample 3: n=16; Mean(pg/ml): 347; Standard deviation: 23.25; CV(%): 6.7

Sample 1: n=24; Mean(pg/ml): 65; Standard deviation: 3.77; CV(%): 5.8 Sample 2: n=24; Mean(pg/ml): 191; Standard deviation: 15.1; CV(%): 7.9 Sample 3: n=24; Mean(pg/ml): 386; Standard deviation: 27.02; CV(%): 7

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of rat IL-3 in cell culture supernates and serum.

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat IL-3 antibody 1 ST0000-10 Lyophilized recombinant rat IL-3 standard 10ng/tubex2 AR1107 Biotinylated anti- rat IL-3 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5.5 The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.

IL3

Interleukin-3

interleukin 3

Burst promoting activity Colony stimulating factor multiple Eosinophil colony stimulating factor Hematopoietic growth factor IL 3 IL-3 IL3 IL3_HUMAN Interleukin 3 Interleukin-3 Mast cell growth factor MCGF Multi CSF Multilineage colony stimulating factor Multipotential colony stimulating factor Multipotential colony-stimulating factor P cell stimulating factor P-cell-stimulating factor Thy1 inducing factor

P04823