product summary
request information :
company name :
Boster Immunoleader
product type :
ELISA/assay
product name :
Human DKK-3 PicoKine ELISA Kit
catalog :
EK1323
quantity :
1 x96 well plate
price :
369 USD
product information
Product Name :
Human DKK-3 PicoKine ELISA Kit
Catalog Number :
EK1323
Price :
369 USD
Description short :
Sandwich ELISA kit for Quantitative Detection of Human DKK-3
immunogen :
NSO, A22-I350
Pack Size :
1 x96 well plate
Assay Range :
156pg/ml-10,000pg/ml
Sensitivity :
<10pg/ml
Intra-Assay Precision :
Sample 1: n=16; Mean(pg/ml): 1228; Standard deviation: 79.82; CV(%): 6.5 Sample 2: n=16; Mean(pg/ml): 3386; Standard deviation: 169.3; CV(%): 5 Sample 3: n=16; Mean(pg/ml): 5833; Standard deviation: 332.5; CV(%): 5.7
Inter-Assay Precision :
Sample 1: n=24; Mean(pg/ml): 1439; Standard deviation: 110.8; CV(%): 7.7 Sample 2: n=24; Mean(pg/ml): 3627; Standard deviation: 196; CV(%): 5.4 Sample 3: n=24; Mean(pg/ml): 6294; Standard deviation: 428; CV(%): 6.8
Cross Reactivity :
There is no detectable cross-reactivity with other relevant proteins.
Storage :
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Sample Type :
This kit is used for quantitative detection of human DKK-3 in cell culture supernates, serum and plasma(heparin, EDTA).
Reactivity :
Human
Kit Components :
Catalog number Description Quantity - 96-well plate precoated with anti- human DKK-3 antibody 1 ST0000-10 Lyophilized recombinant human DKK-3 standard 10ng/tubex2 AR1107 Biotinylated anti- human DKK-3 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml
Background :
Dickkopf-related protein 3 is a protein that in humans is encoded by the DKK3 gene. This gene encodes a protein that is a member of the dickkopf family. It is mapped to 11p15.3. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Members of the Dkk-related family display unique patterns of mRNA expression in human and mouse tissues, and are secreted when expressed in 293T cells. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Gene Name :
DKK3
Protein Name :
Dickkopf-related protein 3
Gene Full Name :
dickkopf WNT signaling pathway inhibitor 3
Synonyms :
AW061014 C87148 Dickkopf (Xenopus laevis) homolog 3 Dickkopf 3 Dickkopf homolog 3 (Xenopus laevis) Dickkopf related protein 3 HDkk 3 Regulated in glioma REIC RIG RIG like 5 6 RIG like 7 1
Uniprot IDs :
Q9UBP4
company information
Boster Immunoleader
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
www.bosterbio.com
925.485.4527
headquarters: USA
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