Boster Immunoleader

ELISA/assay

Mouse CD169/SIGLEC-1 PicoKine ELISA Kit

EK1305

1 x96 well plate

369 USD

Mouse CD169/SIGLEC-1 PicoKine ELISA Kit

EK1305

369 USD

Sandwich ELISA kit for Quantitative Detection of Mouse CD169/SIGLEC-1

NSO, T20-R1639

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 1.64; Standard deviation: 0.06; CV(%): 3.7 Sample 2: n=16; Mean(ng/ml): 4.85; Standard deviation: 0.204; CV(%): 4.2 Sample 3: n=16; Mean(ng/ml): 6.88; Standard deviation: 0.316; CV(%): 4.6

Sample 1: n=24; Mean(ng/ml): 2.54; Standard deviation: 0.119; CV(%): 4.7 Sample 2: n=24; Mean(ng/ml): 5.31; Standard deviation: 0.27; CV(%): 5.1 Sample 3: n=24; Mean(ng/ml): 7.05; Standard deviation: 0.437; CV(%): 6.2

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse CD169 in cell culture supernates, serum and plasma(heparin, EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse CD169 antibody 1 ST0000-10 Lyophilized recombinant mouse CD169 standard 10ng/tubex2 AR1107 Biotinylated anti- mouse CD169 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily(IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.

SIGLEC1

Sialoadhesin

sialic acid binding Ig-like lectin 1, sialoadhesin

CD169 CD169 antigen dJ1009E24.1 DKFZp667F058 FLJ00051 FLJ00055 FLJ00073 FLJ00411 FLJ32150 Sialic acid binding Ig like lectin 1 Sialic acid binding immunoglobulin like lectin 1 Sialic acid-binding Ig-like lectin 1 Sialoadhesin Siglec 1 Siglec-1 Siglec1 SN SN_HUMAN

Q62230